| Iron is an essential component of numerous proteins and enzymes, the number and diversity of enzymes that contain or depend on the presence of iron for activity is extensive in human body. In recent years, the deleterious of iron has been given more and more attention, and many studies have focused on the reactive oxygen species induced by excess iron, which can induce the oxidative injury. Liver, heart and kidney are the main organs to be injured when iron overload occurs. Flavonoids are a group of natural antioxidants, which have been researched in antioxidant and pharmacological effects for many years. In this thesis, the protective effects of baicalin on iron induced HepG2 cell oxidative injury were studied, the result showed that baicalin have good effects of anti-toxicity to iron overload. The main results are as follows:①The MTT method was used to studied the cytotoxicities of hemin, ferritin, hemoglobin, ferric citrate, EDTA-Fe(III) at different concentration as well as the combination of those iron compound with nitrite and glucose oxidase(GO) on HepG2 cell. The results showed that all Fe(III)-nitrite-GO system could effectively decrease HepG2 cell viability. Hemin-nitrite-GO system was the most effective one.②We also studied the protective effect of baicalin on ferritin -nitrite-GO and ferric-nitrite-GO induced liver cell line HepG2 injury. The results showed that baicalin could effectively inhabited ferritin/ferric citrate-nitrite-GO systems induced cell oxidative injury, but in the concentration of 5 ~ 45μmol/L, lower dose showed a better protection manner.
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