| Objective Polyethylene glycol-bovine (PEG-bHb) is a kind of hemoglobin-based oxygen carriers. Mixing St. Thomas cardioplegic solution with PEG-bHb makes a "blood-containing cardioplegia". The aim of the present study was to investigate the effect of PEG-bHb-containing cardioplegic solution on the protection of isolated rat heart, with the comparison of St. Thomas solution. The results might demonstrate that its oxygen-delivering property could effectively preserve ischemic heart during cardioplegia.Methods 32 male S-D rats were divided into 4 groups according to cardioplegic solutions: Group A, St.Thomas solution; Group B, 1:2 PEG-bHb, Group C, 1:4 PEG- bHb, and Group D, 1:8 PEG-bHb (The term 1:X meant PEG-bHb with 15%KCl 10ml/L in it were mixed with St. Thomas solution at the ratio of 1 :X). Hearts of rats were harvested after anesthesia and rapid chest opening, and were transferred to Langendorff circuit. After 20min balance period, hearts were perfused with cold (4℃) cardioplegic solutions, and preserved at 30℃for 60min, then reperfused. Left ventricular developed pressure (LVDP), derivative of pressure (± dp/dtmax) and coronary flow (CF) were recorded. Myocardial water content, ATP content and cardiac troponin I (cTnl) in coronary effluent were detected. The ultrastructures were also observed. Data were treated by variance analysis of repeated data or one-way ANOVA .Results After reperfusion, left ventricular developed pressure (LVDP), derivative of pressure (±dp/dtmax), coronary flow (CF) and ATP content in Group B, C and D were higher than those in Group A (P<0.05) . Cardiac troponin I (cTnI) were lower in Group B, C and D than in Group A (P<0.05) . Water content were lower in Group B and C than in Group A(P<0.05). The ultrastructures were preserved better in Group B, C and D. There was no difference among Group B, C and D (P>0.05) except for CF.Conclusion PEG-bHb in cardioplegic solutions can provide better myocardial protection during ischemia than St. Thomas solution.
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