Comparison Of Recombinant Tissue Plasminogen Activator, Urokinase And Defibrase In Vitro Simulation Of Thrombolytic Test

ObjectiveTo determine the effect of recombinant tissue plasminogen activator(rt-PA), urokinase(UK) and defibrase in vitro.MethodsClots were made in vitro using the blood of a healthy volunteer,grouped into four groups,and sent to the homoeothermic and constant flow system which contained different drugs and normal saline.D-dimmer(DD) contents of the fluid and the weight of the clots at 1,2 and 3 hours after drug administration were detected respectively. Finally,the percentages of weight reductions and the D-dimmer contents at at 1,2 and 3 hours after drug administration were compared respectively.ResultsThe percentage of weight reductions at 3 hours in rt-PA group was significantly higher than that in UK group(93.74%±4.83%VS 81.82%±4.26%,P=0.030),and the percentages of weight reductions at 3 hours in UK group was significantly higher than that in defibrase group(81.82%±4.26%VS 26.41%±3.94%,P=0.000),while there was no significant difference between defibrase group and blank control group (26.41%±3.94%VS 26.06%±5.11%,P=1.000).Comparision of the D-dimer contents at 1,2,3 hours after drug administration showed that the D-dimer contents in the rt-PA group was significantly higher than UK group(P＜0.05) at each time point,and the D-dimmer contents in UK group and defibrase group were undetectable;the D-dimmer contents in rt-PA group and UK group both increased faster during the first hour than that during the second and the third hour(725.01±791.30ng/ml VS19329.21±625.75ng/ml).ConclusionThrombolytic effects of rt-PA and UK are definite;rt-PA has a higher thrombolytic rate than UK,Defibrase has no effect of thrombolysis.