Research Of Mitochondrial DNA T1095C Genovariation In Military Noise-induced Hearing Loss

Objective:Mutations in mitochondrial DNA(mtDNA),particularly those in the 12SrRNA gene,have been shown to be associated with military noise-induced hearing loss.Here we analyzed the clinical and sequence analysis of the enire mitochondrial genome in five subjects with military noise-induced hearing loss.Method:we collect 304 soldiers who exposed to military noise in Yunan and Beijing, including susceptible and tolerant groups.Genomic DNA was isolated,we found mitochondrial 12SrRNA T1095C mutation in 5 subjects.Then we processed the complete nucleotide sequence to five subjects,at the same time,analysed these clinical characters.Result:we found that 5 subjects have taken mitochondrial 12SrRNA T1095C mutation in 165 in experimental group.Besides the subjects both were distributed in experimental groups(susceptible groups),through X~2 test,P＜0.05,we think that there are distinct contrasts in two groups.Five soldiers both represent different degrees sensorineural deafness who exposed to military noise.Sequence analysis of the complete mitochondrial genomes in five subjects showed the distinct sets ofmtDNA polymorphism,in addition to the identical mitochondrial 12S rRNA T1095C mutation.Conclusion:In fact,the occurrence of the T1095C mutation in these several genetically unrelated subjects who exposed to military noise affected by hearing impairment strongly indicates that this mutation is involved in the pathogenesis of hearing impairment.We indicate that the T1095C mutation can be pathogenic mutations associated with NIHL.