Study On Ad-hTERTp-HSV-TK/GCV Therapy In Combination With Oxaliplatin On Human Hepato Carcinoma Cells

Objective To evaluate the lethal effect of adenovirus-mediated Ad-hTERTp-HSV-TK/GCV suicide gene therapy in combination with oxaliplatin(L-OHP) on human hepato carcinoma cell line HepG2 in vitro.Methods We used the replication-defective adenovirus carring the HSV-TK gene under control of the hTERT promoter to transfer the HepG2 cells in vitro.Human hepato carcinoma cell line HepG2 was transfected with MOI=100.Study the grow inhibitory effction which Ad-hTERTp-HSV-TK/GCV therapy,oxaliplatin Ad-hTERTp-HSV-TK/GCV therapy in combination with oxaliplatin through different drug concentration on human hepato carcinoma cell line HepG2.The growth inhibition rate of HepG2 cells was determined by trypan blue exclusion assay and MTT.Results The growth of HepG2 cells Ad-hTERTp-HSV-TK/GCV,oxaliplatin,and combination of Ad-hTERTp-HSV-TK/GCV and oxaliplatin was significantly slower.The more concentration the greater inhibition of cell growth.The growth inhibition rate of combined Ad-hTERTp-HSV-TK/GCV with oxaliplatin is 88.42%.The growth inhibition rate of Ad-hTERTp-HSV-TK/GCV is 82.87%.The growth inhibition rate of oxaliplatin is 73.43%. combination of Ad-hTERTp-HSV-TK/GCV and oxaliplatin resulted in greater inhibition of cell growth combinated therapy compared with TK gene and L-OHP(P<0.05).Conclusion HSV-TK/GCV in combination with L-OHP can enhance the lethal effect of suicide gene therapy against HepG2 cells.It is more targetable than the function of single drug therapy.Also,it could reduce drug level and plays an important role on the future's clinical medication.