Protection Mechanisms Of Porphyra Polysaccharide On Gastric Mucosa Avoiding Acute Damage Of Alcohol In Mice

Objective : To study the protection mechanisms of porphyra polysaccharide on gastric mucosa avoiding acute damage of alcohol in mice.Methods: Mice were divided into saline group, model group, cimetidine group and low dose, medium dose, high dose porphyra polysaccharide group. Each mouse was administered with 1ml/kg saline in saline group and model group, 100mg/kg porphyra with the concentration of 1% in low dose porphyra polysaccharide group, 200mg/kg porphyra with the concentration of 2% in medium dose porphyra polysaccharide group, and 400mg/kg porphyra with the concentration of 4% in high dose porphyra polysaccharide group. Once a day for 8 days. On 8th day in each group, Fasting was did for 24h after administration. On 9th day, each mouse in every groups except saline group was administered with 0.2ml ethanol after 30min of administration. Mice were executed 1h later, then, taked out gasters and observed the situation of gastric mucosal damage and ulcer, maked homogenate with whole gasters, detected contents of malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO) and prostaglandin (PGE2) homogenate of the gaster.Results: Ulcer indexes and contents of MDA in high, medium and low-dose porphyra polysaccharide group were lower than that in model group. Contents of SOD, NO and PGE2 in high, medium and low-dose porphyra polysaccharide group were higher than that in model group, and porphyra polysaccharide had some dose dependent(P<0.05,P<0.01).Conclusion: Porphyra polysaccharide has a protective effect on gastric mucosal avoiding acute damage of alcohol in mice. Protection mechanisms may be related to SOD, NO and PGE2.