The Level And Meaning Of Chemokines CXCL-9 And CXCL-10 In Pleural Effusion

Objective:The aim of the present study was to investigate the concentrations and clinical significance of CXCL-9 and CXCL-10 in pleural effusions(PE) with various etiologies.Methods:Pleural effusion samples were obtained from 88 patients who presented to the respiratory institute(32 with tuberculous pleural effusion,30 with malignant pleural effusion,15 with bacterial pleural effusion,and 11 with transudative pleural effusion).The pleural effusion and serum levels of CXCL-9 and CXCL-10 were determined by sandwich enzyme-linked immunosorbent assays(ELISA).Results:1.Therewas statistical significance in pleural concentrations of CXCL-9 and CXCL-10 between the exudative groups and transudative groups (P＜0.05);The concentrations of CXCL-9 in tuberculous PE(2141.52 (1922.61)pg/ml)was significantly higher than that in malignant group(290.94 (408.45) pg/ml).but there was no statistical significance in the concentrations of PE CXCL-9 in tuberculous group and bacterial group,Neither in malignant group and bacterial group.There was statistical significance in pleural concentrations of CXCL-10 between the tuberculous groups and bacterial groups(P＜0.05),the concentrations of CXCL-10 in tuberculous PE(545.24 (184.70) pg/ml) was higher than that in bacterial group(340.70 (263.09) pg/ml);there was also statistical significance in pleural concentrations of CXCL-10 between the malignant groups and bacterial groups (P＜0.05),the concentrations of CXCL-10 in malignant PE(497.24(199.02) pg/ml) was higher than that in bacterial group(340.70(263.09)pg/ml);but there was no statistical significance in the concentrations of PE CXCL-10 in tuberculous group and malignant group.2.Between the tuberculous group and malignant group,the concentrations of CXCL-9 and CXCL-10 in serum both indicated statistical significance(P＜0.05),the concentrations of CXCL-9 in tuberculous serum(241.27(304.42) pg/ml) was higher than that in malignant group(120.96(140.78) pg/ml),the concentrations of CXCL-10 in tuberculous serum(197.18(199.70) pg/ml) was higher than that in malignant group(114.60 (91.17) pg/ml).3.Between PE and serum,there was statistical significance in each of exudative groups to the concentrations of CXCL-9 and CXCL-10,but not in transudative groups.4.The pleural levels of CXCL-9 and CXCL-10 was positive correlated with the respongding serum levels(p＜0.05),the coefficient of correlation was 0.431 and 0.271 respectively;In PE,CXCL-9 was positive correlated with CXCL-10(p＜0.05,r=0.474),also in serum(p＜0.05, r=0.717).5.The cut-off value of pleural CXCL-9 for the diagnosis of tuberculous PE was 147.39 pg/ml with the corresponding sensitivity and specificity 0.867 and 0.875,respectively.Conclusions:Chemokines of CXCL-9 and CXCL-10 were likely to participate in the inflammatory response of pleurisy.Pleural CXCL-9 may be helpful for the differential diagnosis of tuberculous and malignant PE,And Pleural CXCL-10 may be helpful for the differential diagnosis of tuberculous and bacterial PE,the same of malignant and bacterial PE.Besides,The detection of pleural CXCL-9 could be a new tool to diagnose tuberculous PE.