Investigation Of XRCC1 And MGMT Expression In Esophageal Carcinoma

OBJECTIVE: In this study, we detected the protein expressions of repair gene MGMT and XRCC1 in esophageal carcinoma, Barrett'esophagus and normal epithelium to analyze the role of their abnormal expressions in esophageal tumorigenesis and provide new theoretical foundation for forecasting the early diagnosis,prevention and clinical treatment,judging prognosis of esophageal carcinoma.METHODS: Expression and their relationship with clinicopathological parameters of MGMT gene and XRCC1 proteins was detected by immunohistochemical techniques in esophageal carcinoma, Barrett'esophagus and normal epithelium.RESULT: The positive expression rate of XRCC1 in esophageal carcinoma being 89.2% was significantly higher than those in Barrett'esophagus being 59.1%and in normal epithelium being 57.1%(P<0.05), while there was no significantly difference between the two later(p>0.05). And there is no obvious correlation between the expression of XRCC1 protein and age, sex, tumor location,infiltrating depth, lymph nodes metastasis and so on. The expression rate of MGMT in esophageal carcinoma being 46.2% was lower than those in Barrett'esophagus being 77.2%and in normal epithelium being 85.7%, while there was no significantly difference between the two later(p>0.05). And there were significant differences about the expression of MGMT in the clinicopathological factors, such as differentiation degree, infiltrating depth, lymph nodes metastasis and so on. The expressions of MGMT had no relationship with sex,age and tumor location. The expression of XRCC1 and MGMT in esophageal carcinoma has no relashionship.CONCLUSION: The abnormal expression of XRCC1 and MGMT genes show that they may play important roles in carcinogenesis and progression of esophageal carcinoma. The expressions of MGMT were significantly correlated with lymph node metastasis and infiltrative deepness(P<0.05), MGMT can become one of the indexes predicting malignant biological behaviour of esophageal carcinoma. The expressions of the expression of XRCC1 and MGMT were not related to each other, they have unattached function.