| Objective: To monitor the improvement of riboflavin and observe the status of mucous membrane of esophagus, so as to provide a local evidence for the generalization of the riboflavin-fortified salt. Based on the effect of riboflavin-fortified salt popularization to prevent the occurrence of esophageal cancer, we carried out an experiment in vitro to research how riboflavin influence the growth of esophageal cancer cell, so as to explore the meaning of maintaining the normal nutritional level of riboflavin in body of esophageal cancer patient.Methods:1 Riboflavin-fortified salt had been fixed-point sold in 9 villages of Guyi commune, while sales of ordinary commercial salt in other 12 hamlets. 10382 Residents who consumed Riboflavin-fortified salt were set up as the intervention group, 10711 residents who consumed ordinary commercial salt were set up as the control group.⑴1036 subjects whose age from 40-69 years old (including 587 in the control group, 449 in the intervention group) were examined esophagus and the entire stomach mucosa by fiberoptic gastroscope, meanwhile biopsy was taken from every subject for pathological diagnosis.⑵Compare the nutritional status of riboflavin by measure the erythrocyte glutathione reductase activity coefficient(EGRAC) in the randomized sample (including intervention group 155, control group 120).⑶Collected 18 blood samples of cancer patients, measure the value of EGRAC. In nested case-control study, consider the 54 residents' EGRAC as the dependent variable, after single-factor analysis of screening, imported the smoking consumpton, alcohol consumption, pickled food consumption in week, blanching eating habit, family history of cancer, etc.2 Conventional cultured human esophageal cancer cell line Eca109.⑴Measured proliferation activity of Eca109 after treated with different concentration of 4nmol/L, 20nmol/L, 100nmol/L, 500nmol/L, calculated the proliferation rate of cells.⑵Determinated cell cycle changes of Eca109 treated with different concentration of riboflavin by FCM.⑶Mensurated the effect of Cyclin D1 protein expression in Eca109 cell treated 24h with riboflavin mensurated by immunohistochemical method.⑷Observed the differentiation influence of Eca109 treated 24h with riboflavin by HE staining.⑸SPSS 11.5 for windows was used for statistical analysis. One-way ANOVA and LSD were used to analyze all experimental data. Comparison of rates was determined using chi-square test. A values of p<0.05 were considered statistically significant.Results:1⑴Gastroscopy showd that the mucosal status of intervention group and control group is: 83.85%and 83.69% normal,15.81% and 13.82% atypical hyperplasia, 1.34% and 2.59% cancer, respectively. The esophageal mucosal status of intervention group is better than control group.(χ2=6.547, P=0.038).⑵The EGRAC of intervention group is 1.452±0.297,lower than 1.606±0.273 of control group, the difference if significant (t=4.412, P<0.001).⑶Family history of cancer (OR=20.94, 95%CI=1.37~ 321.28) and lack of riboflavin (OR=15.51, 95%CI=1.54~ 156.08) might be a risk factor for esophageal cancer, and even there is interaction between them.2⑴Generally speaking, the growth curve of the CEA109 treated with different riboflavin concentration showed upwad trend with time:①After treated with riboflavin 12h, there was no significant difference between each two riboflavin treated Eca109 groups excpet of 20nmol/L and 100nmol/L, and no significant difference between riboflavin treated group and negative control group (P>0.05).②After treated with riboflavin 24h, the growth rate of groups with 0, 4, 20nmol/L riboflavin concentration were significantly different from 500nmol/L (P<0.05) except of 100nmol/L (P>0.05).③After treated with riboflavin 36h, there was no significant difference between each riboflavin-treated groups and negative group (P>0.05), while they were all significantly different from positive group (P<0.05).④After treated with riboflavin 36h, there was no significant difference between each riboflavin-treated groups and negative group (P>0.05), while they were all significantly different from positive group (P<0.05).⑵Cell cycle of Eca109 was blocked in M phase and no apoptosis was detected.⑶After treated with riboflavin 24h, the expression of Cyclin D1 protein didn't show lower than negative group or 5-Fu group (P>0.05) in Eca109 by immunocytochemistry.⑷Observed cell morphous of each goup by HE staining.Conclusions: Riboflavi-fortified salt can enhance the nutrition status of riboflavin, and further improve the esophagus mucosal health status, furthermore, riboflavin deficiency might be one of the risk factors in this high incidence area. Maintain the plasma riboflavin concentration between 5-50nmol/L might not influence the proliferation of Eca109 cell, otherwise, cell growth can be block in the M phase in the cell growth cycle in vivo. |
PDF Full Text Request