Study On The Mechanisms Of Cisplatin Induced Apoptosis And Drug Resistance In IGROV-1 Ovarian Cancer Cells

Cisplatin (PC) is a drug that is often used for clinical chemotherapy of ovarian cancer. Human IGROV-1 cell line was used as a model to explore the molecular mechanisms of PC induced apoptosis and drug resistance in this study. First, miRNA expression map was constructed using the sequencing by synthesis (SBS) based Illumina sequence analysis platform. The acquired sequences were filtered using parameters : length difference≤2 and no mismatched base as compared with miRNA sequences in the database. 53 known miRNAs were found, 56.6% miRNAs are of low relative abundance; their sequences were repeatedly observed within less than ten times during sequencing. Second, TaqMan based real-time RT-PCR were used to compare the expression of 5 genes in IGROV1 PC-sensitive and IGROV1/CP PC-resistant ovarian cancer cells, the protein products of which have been reported to be correlated with development of PC-resistance. It was found that the differential expression of AK1 and GALT mRNAs were in accordance with their proteins'expression. Last, gene expression microarray was employed to probe PC induced changes of gene expression profiles of IGROV1 cells. 836 genes were found to be differentially expressed after 48 hours of PC treatment. 15 genes were selected for quantitative real-time RT-PCR analyses. Among them, 8 genes were found to be differentially expressed; this corroborated the data obtained by array analysis. Through the studies as above, some important genes were identified to be related with PC-induced apoptosis and possibly the development of PC-resistance. All these results are good basis for elucidating the underlying molecular mechanisms.