Evaluation Of Cytocompatibility The Biodegradable Polyhydroxybutyrate By Ames Test

ObjectiveOne of the effective ways to improve the corrosion resistance of aluminum alloy is providing the surface of magnesium matrix with a layer of corrosion,which can be regarded as a corrosion barrier.Based on the modern surface technology,as well as the requirements of environmental protection,micro-arc oxidation,aluminum coatings, aluminum fluoride coatings,fluoride concord coating,vapor deposition,ion implantation and other new technologies in improving the corrosion resistance of aluminum-magnesium are increasingly popular.And plolyhydroxybutyrate layer is a significantly new promising technology.It,widely existing in many natural prokaryotes, is a natural polymer stored in cells,and it is formed by a kind of microorganism growing in imbalance growth condition.Plolyhydroxybutyrate is now widely used in drug delivery and tissue engineering.On the basis of ISO correlated standardization, leaching liquor of biodegradable polyhydroxybutyrate was made out to progress Salmonella typhimurium reverse mutation assay(Ames test) in this study.And the purpose of this study was to answer the question whether the biodegradable polyhydroxybutyrate had potential mutagenicity and carcinogenicity.Methods1,Appreciation of stain genetic determinantSix stain genetic determinant experiments include:the requirement of histidine, discrepancy of lipopolysaccharides barrier,repair-deficiency of DUV,test of anti-AMP, test of anti-TCN and spontaneous reverse assay were carried out.After the appreciation of stain genetic determinant,incubation at 37℃,make bacterium fluid density to 1-3×10~9/ml.2,Nutrient mediumFour kinds of nutrient mediums were made reference to Salmonella typhimurium reverse mutation assay.3,Activation systemS-9 and S-9mix were made reference to Salmonella typhimurium reverse mutation assay.4,Design of dosageClean the sample alloy plate with alcohol and supersonic,sterilizing,use normal sodium as diffusion medium,in accordance with GB/T16886.12-2000,establish standard leaching(liquorstock suspension) by means of diprosopia surface area/ diffusion medium is 3cm~2/ml.1/2,2 and 4 times of liquorstock suspension were prepared.37℃±1℃,24h±2h diffused.5,Salmonella typhimurium reverse mutation assayIn the flat-plate incorporation test of Salmonella typhimurium,the average number of spontaneous revertants of TA97,TA98,TA100,TA102,TA1535 under 4 concentrations was calculated after incubation at 37℃for 48 hours.ResultsThe number of induced revertants when compared to that of spontaneous revertants was not reproducibly higher in a dose-related manner.The result of Ames test for biodegradable polyhydroxybutyrate was negative.ConclusionIt implies biodegradable polyhydroxybutyrate has no potential mutagenicity.