The Molecular Mechanism Aralia Elata Seem Leaves On Anti-Tumor

Objective: To explore the effect and molecular mechanism in anti-tumor of Aralia elata Seem Leaves. To provide a reliable scientific basis in providing a newer anticancer drug target. To lay a solid foundation for research and development of anti-tumor drugs with independent intellectual property rights. Providing a new way of thinking and methods for the anti-tumor mechanism of traditional Chinese medicine .Methods: (1) Sulforhodamine B dectects the inhibition effect on A549,BGC,BEL-7402,BGC cells by using the total saponins of Aralia elata Seem Leaves. Every tumor cell is detected by inverted fluorescence microsope to know how the grow.(2)A549 tumor model is established on nu/nu mouse in order to carry through restaining tumor experiment in mouse body. After model was established ,the mice were subgroups 3 groups by stochastic method according to the murine body weight.After 10 weeks, we can take out of the tumor-body, then we will weigh the tumor-body's weight.,at the same time, calculate the inhibition ratio of the tumor. At equal pace,we will put the tumor-body into 10% formaldehyde-PBS solution,two-step immunohistochemical method detect the correlative protein COX-2 in the tumor-body and detect the MVD by CD31 antibody.Results: (1)ETS can restrain the growth of A549,BGC,BEL-74-2 and HCT-8 cells in 96 Well Culture Cluster.(2)ETS can restrain the growth of A549 tumor model in nu/nu mouse body.(3)ETS can restrain the expression of COX-2.(4)ETS can restrain the growth of the MVD in tumor-body.