The Influence On Na~+-K~+-ATP Enzyme Activity In Red Blood Cell Of Jcd To Dpn Wistar

Objective:To study and research the therapeutic action of JCD to DPN Wistar rat,Observe the influence to Wistar rats' serum glucose, blood-serum-insuline,blood-serum-C-peptid level,MNCV,pathomorphology change of sciatic nerve and Na~+-K~+-ATP enzyme activity in RBC.Approach DPN pathogenesy further.To develope the Chinese drugs preparation of efficaciously treat DPN.Methods:Select healthy Wistar rats(body weight200g±20g)90 cases. Adaptivity feeding one week.Choose 10 cases randomly as normal control. The rest 80 cases used for making mode.After absoluting diet 12 hours, Successuflly reconstructed the model of DPN after injecting the Wistars'left lower-abdomen with streptozotocin(STZ) onetime.We divided the models into 6 groups randomly.They are model group,high,middle and low dosage of JCD group,Erjiashuanggua group and Mikebao group.There were 10 in each group.After 8 weeks,we observed the effect of JCD on serum glucose, blood-serum-insuline,blood-serum -C- peptid level,MNCV, pathomorphology change of sciatic nerve and Na~+-K~+-ATP enzyme activity in RBC on model rats by the technic methods of Eilectrophysiology,Pathology and so on.Results:1.JCD Could significantly decrease DPN serum glucose,increase blood-serum -insuline and blood-serum -C- peptid level.The high group of JCD could get the best result.2.JCD could rise MNCV of DPN rats greatly and relieve the damage of sciatic nerve in Pathology,in which the high group of JCD could get the best result.3.JCD could rise the Na~+-K~+-ATP enzyme activity in RBC of DPN rats greatly in which the high group of JCD could get the best result.Conclusion:JCD could prevent and cure DPN on the followings:decrease serum glucose,increase blood-serum -insuline and blood-serum -C- peptid level,rise MNCV,relieve the damage of sciatic nerve in Pathology,rise the Na~+-K~+-ATP enzyme activity in RBC.