Investigation On Mosquitoes In Xiamen And Studies On The Establishement Laboratorial Population,Biological Characteristics And Acute Toxicity Test Of Aedes Albopictus

Blood-sucking mosquitoes of Diptera(Culicinae) in Xiamen were investigated during Apr.2008-Nov.2008.6 species of mosquitoes belonging to 2 families,2 orders were collected,observed and identified.These species are:Culex fatigans Wiedemann,1828Culex tritaeniorhynchus Giles.1901Culex mimeticus Noé,1899Aedes albopictus(Skuse),1894Aedes macfarlanei,1914Anopheles(A.) hyrcanus var.sinensisThe laboratory population of Xiamen strain of Aedes albopictus had been successfully established.We explored out favorable conditions and reproduced them 6 generations.Biological morphological characters of the whole life cycle stage were described, and egg hatching,pupation,emergence were also observed and analyzed.Egg hatching rate of Xiamen strain of Aedes albopictus was high and stable;The average survival rate of larvae was 98.2%;the pupation rate was higher than 90%and emergence rate was more than 97.2%.Male Aedes albopictus can survive 14 days to 38 days,and females can survive 20 days to 62 days.Aedes albopictus prefers to lay eggs on ovipositor whose surface is much rougher, and whose background is much darker when we studied on its spawning habit.Last,we used Cu²⁺,Hg⁺ and Sn(CH₃)₃ to learn acute toxicity test results of the second-stage,forth-stage larvae and egg of Aedes albopictus in different doses.Median lethal concentrations(LC50 values) were obtained using Probit method.For second-stage of Aedes albopictus exposed to copper,mercury and trimethyltin,the 50% lethal concentrations(LC50) after 24h were observed respectively to be 175.67,10.47,2.19 mg·L^-1,and the forth stage larvae's LC50 after 24h were observed respectively to be 282.42,18.64,2.92mg·L^-1.Egg hatching rate was effected when exposed to Cu²⁺,Hg⁺,Sn(CH₃)₃,and was all lower than control group,but they were not exist differences between each other statistically.Tolerance ability of larvae of Aedes albopictus is different from each other when exposed to different venoms.Acute toxicity of Sn(CH₃)₃ on larvae of Aedes albopictus is greater than that of Hg⁺and Cu²⁺,and that of Cu²⁺is the lowest.LC50 values of different stage larvae is different when exposed to the same venom, and tolerance ability is also different.