Anti-Epileptic Effects Of Curcumin And Its Possible Mechanisms

Objectives:(1) To indentify the neuroprotection of curcumin against pilocarpine-induced-seizure-making;(2) To elucidate the possible effect of curcumin on the electrophysiological property of Na/K-ATPase,EAAC1 and GAT1.Methods:(1) Pilocarpine-induced animal model of epilepsy was built and the manifestation of the epilepsy rats,with or without curcumin pretreatment,was recorded and assessed.(2) The Xenopus Laevis oocytes were utilized as a protein expression model and heterogeneous cRNA of SD rats were expressed in the oocytes. Two-electrode voltage clamp was employed to detect the steady-state current induced by these proteins.Curcumin was administered to the oocytes via different methods respectively,perfusion,incubation or intracellular injection,for example.The changes before and after the treatment of curcumin were observed.Results:(1) Both models of epilepsy and the heterogeneous protein expression were successfully built;(2) Curcumin could alleviate the epileptic seizures of the pilocarpine-induced model of epilepsy;(3) Application of a series list of curcumin (7.SUM,15uM,30uM and 75uM) could not affect the stead-state current induced by Na/K pump.Neither could the protocol of incubation nor intracellular injection.(4) Current of EAAC1 was not sensitive to curcumin(30uM) either.(5) The current of GAT1 was slightly reduced after the treatment of curcumin.Conclusion:(1) Pretreatment of curcumin intraperitoneally could protect animals from epilepsy induced by pilocarpine.(2) Curcumin could not affect the current induced by Na/K-ATPase or EAAC1,but could slightly suppress the current induce by GAT1.