Research On Expression Of Twist Gene In Colon Carcinoma

Objective:To detect the expression of Twist gene in colon carcinoma tissue and study the association of the changs in tumor differentiation,invasion,lymph node metastasis,metastais and TNM staging of the colon carcinoma.To explore the relationship between Twist gene expression and tumoregenesis,development in colon carcinoma.Methods:The tumor and cutting edge tissues were gained from 50 cases of colon carcinoma specimens which were collected from surgical resection.Real-time quantitative polymerase chain reaction(real-time PCR) was used to detect the expression of Twist mRNA.Streptavidin biotin complex(SABC ) immunohistochemistry was performed to detect the Twist protein expression.Results:1.The expression of Twist mRNA in colon carcinoma tissues is higher than that in cutting edge tissues(P<0.05),Twist gene mRNA transcription in colon carcinoma had statistical significance in different tumor differentiation,invasion depth,lymph node metastasis,metastasis and TNM staging respectively(P<0.05).There were no statistical significance in age and gender and pathological type(P>0.05).2.The positive rates of Twist protein expression in colon carcinoma and that in cutting edge tissues were 68%and 16%,the Twist protein expression in colon carcinoma tissues is higher than the level in cutting edge tissues(P<0.05).Twist protein expression in colon carcinoma also had statistical significance in different tumor differentiation,invasion depth,lymph node metastasis,metastasis and TNM staging respectively(P<0.05).There were no statistical significance in age and gender and pathological type(P>0.05).3.The Twist mRNA transcript expression had a positive correlation with protein expression. Conclusions:1.The high expression of Twist gene plays an important role in tumoregenesis and development of colon carcinoma.Twist gene acts as one of the characteristics of molecular biology in the process of malignant transformation of colon tumor.2.The mRNA transcript of Twist gene is positively correlated with its corresponding protein expression in colon carcinoma,indicating that the activation of Twist gene is not only play an important role in the mRNA transcription level,but also in the protein level.3.The correlations between expression of Twist gene and pathlogical parameters shows that The activation of Twist gene is likely to be an important event in invasion and matastasis of colon carcinoma.4.The strategy that Twist gene as a gene therapeutic approach will probably become a kind of mean of treatment in colon carcinoma.