Research On The Expression Of CCL25/CCR9 In The Patients Of Pancreatic Cancer

OBJECTIVETo investigate the expression of CCL25 and CCR9 in pancreatic cancer and tumor draining lymph nodes(TDLNs) at level of mRNA and protein.Then to seek the relationship between the expression of CCL25/CCR9 and the prognosis of pancreatic cancer.Detect the expression of MMP2,to determine the mechanisim how CCL25/CCR9 may influence the invasion and metastasis of pancreatic cancer.METHODS34 cases of pancreatic cancer and 10 cases of pancreatic benign diseases were collected from Tianjin medical university cancer hospital,reverse-transcriotion-PCR and were used to detect the mRNA of CCL25 and CCR9 in pancreatic cancer,TDLNs and benign diseases;58 cases of pancreatic cancer and 10 cases of pancreatic benign diseases were collected from Tianjin medical university cancer hospital, Immunohistochemistry was used to detect the expression of MMP2 proteins in the these tissue.RESULTSThe mRNA of CCR9 in TDLNs was higher than pancreatic cancer(P＜0.05) and pancreatic cancer was higher than benign diseases(P＜0.05).The same result was found in CCL25.Immunohistochemistry results showed that the positive ratio of CCR9 in TDLNs 86.2%was higher than pancreatic cancer 79.3%(P＜0.05),the pancreatic cancer 79.3%was higher than benign diseases 40%(P＜0.05).The positive ratio of CCL25 in TDLNs,pancreatic cancer and benign diseases were 91.4%(53/58), 62.1%(36/58),40%(4/10).The expression of CCL25/CCR9 protein in pancreatic cancer was associated with clinical stage and pathological type while had no relationship with tumor diameter.The positive ratio of MMP2 in pancreatic cancer 65.5%(38/58) was higher than the benign diseases 40%(4/10)(P＜0.05)and the TDLNs87.9%(51/58) was higher than pancreatic cancer65.5%(38/58)(P＜0.05).The expression of CCL25/CCR9 was positive correlation with the distribution of MMP2 in pancreatic cancer(r=0.435,P＜0.05).There was statistical difference in survival time between CCR9 positive cases and negative cases(P＜0.05),statistical difference in survival time also found between MMP2 positive cases and negative cases(P＜0.05).CONCLUSIONThe expression of CCL25/CCR9 in pancreatic cancer is upregulated.The high expression of CCL25 is accompanied by increasing MMP2 in pancreatic cancer and TDLNs,which suggests that CCL25/CCR9 may involved in pancreatic cancer metastasis.CCR9 may be regarded as an important marker in prognosis judgment. The detection of CCR9 has important significance for clinical therapy,searching the drugs which can block the of interaction of CCR9 and CCL25 may inhibit the invasive growth of cancer.It may offer a new thought to gene therapy for pancreatic cancer.