Influence Of NanoAg Content On The Proliferation Behavior Of HUVEC And HUASMC

Such problems as the restenosis of stent (e.g. Coronary artery stent) and the growth of Tumor are concerned to the Cell hyperplasia, and preferred cell restrain effect of implanted material may provide a way to reduce these problems.Silver has a strong antimicrobial property and has been clinically used as antimicrobial agent such as burn wound dressing in a form of salt (e.g. AgNO₃), compound (e.g. SD-Ag) and nanoAg. During the usage it is found that Ag will interact with cell, which implies that it can be used to restrain cell proliferation. Therefore, the influence of nanoAg on the proliferation behavior of HUASMC and HUVEC was investigated, in order to determine the concentration of nanoAg with which the proliferation of HUASMC rather than HUSMC can be effective restrained. The research result may benifial for the clinc application of nanoAg on its selective restrain effect on cell proliferation.In this paper, the influence of the concentration of nanoAg in a range of 0.015625 to 0.25 mg/mL on the proliferation behavior of HUASMC and HUVEC was in vitro studied via MTT test, BCA assay, and Alarma Blue Assay. By means of P test, one-factor/two factors analysis of variance, the the law of the proliferation law was concluded, and further testified to be liable by IP cell cycle test on FCM. In order to find the reason why the proliferation law is different between two cell lines, the cytotoxicity of nanoAg was studied by LDH assay, the adhesion and aggregation behavior of nanoAg were studied by means of UV spectrophotometer and high resolution TEM.The results show that: the proliferation of both cell lines was restrained by nanoAg. With the increase of the concentration of nanoAg, the restrain effect shows to be nonlinear, and proliferation laws of the two cell lines are different. The proliferation of HUASMC was more significantly restrained with lower nanoAg contration (0.015625 mg/mL), while for HUVEC, it happens with a higher concentration which is 0.25 mg/mL here. The proliferation difference is not caused by the cytotoxicity of nanoAg, but attributes to the adsorption and aggregation of nanoAg in culture medium.