The Study For The Effects Of Fluid Shear Stress On The Cytoskeleton Of The Osteoblast Cells.

AIM : To investigate the effects of fluid shear stress on cytoskeleton of osteoblasts, the authors examined the effects applying confocal laser scanning microscope and Immunofluorescence microscopy. METHODS:The experiments were divided into three groups, I group, fluid shear stress; II group, fluid shear stress +cytochalasinD (CD); III group, fluid shear stress+nocodazole (NO). 12dyne/cm2 fluid shear stress was applied on the osteoblasts at four time points, and cells was marked by FITC-phalloidin and PI double label technique. Images were observed by confocal laser scanning microscope and immunofluorescence microscopy. RESULTS: After the application of fluid shear stress for 1h at 12dnye/cm2, F-actin filaments were organized into stress fibers that became thicker and more abundant than 0 min fluid shear stress cells. Shear stress was associated with significant increases of fluorescence intensity of F-actin. The minimum doses of nocodazole (microtubules) and cytochalasin D (actin filaments) partially disrupted the cytoskeleton. In cytochalasin D-treated cultures, F-actin filaments and the fluorescence intensity of F-actin significant decreased. While, F-actin slightly decreased in nocodazole treatment. CONCLUSION These results indicate that the cytoskeleton, especially F-actin filaments, play a crucial role in cellular mechanotransduction.