Gastric Feeding Salidroside Decoction Antagonist In Rat Liver In Situ Warm Ischemia-reperfusion Injury

ObjectiveHepatic ischemia-reperfusion injury(Hepatic Ischemia Reperfusion Injury,HIRI) is a common clinical pathophysiological process,commonly found in liver resection,hemorrhagic shock,liver transplantation for patients with surgical failure and led to the death of one of the reasons. Rhodiola is for Crassulaceae Rhodiola(RhodiolaL.),widely distributed in China in the northeast,northwest,southwest and other places,the majority of growth in an elevation of 3500-5000m of limestone,granite mountain glaciers,such as extremely poor changing environment.In recent years,scholars have done many aspects on salidroside study,Rhodiola has been found that anti-hypoxia,anti-aging,anti-radiation and a variety of two-way,such as the role of immune regulation.Salidroside this topic through research on rat liver in situ warm ischemia-reperfusion injury of the antagonistic effect,its possible role in the mechanism are discussed.Methods72 Sprague-Dawley adult rats,were randomly divided into:(1) saline control group(A group,n = 24):normal saline 2ml gavage for five days, the last administered 2h after the first hepatic portal resistance Pringle 30min break and then release the non-invasive arterial folder and restore hepatic blood flow,respectively,30min ischemia and after reperfusion 1,6,and24h at each time point based on live animals to kill each observation time point 6 animals;(2) salidroside pretreatment group(B group,n = 24):salidroside 2ml decoction administered 5 days after the last administered 2h after portal triad clamping the first 30min and then Pringle folder to open non-invasive artery to restore blood flow in portal triad,respectively,in ischemia-reperfusion after 30min and 1,6,and 24h at each time point based on live animals to kill each observation time point six animals;(3) Pentoxifylline control group(C group,n = 24): Pentoxifylline 2ml gavage for five days,the last administered 2h after portal triad clamping the first 30min and then Pringle release of non-invasive arterial folder,the restoration of hepatic blood flow, respectively,on ischemia-reperfusion after 30min and 1,6,and 24h at each time point based on live animals to kill each observation time point six animals.Heart blood,AU400 automatic biochemical analyzer determination of alanine aminotransferase(ATL) and aspartate aminotransferase(AST) changes.Homogenate of liver tissue were used thiobarbituric acid colorimetric determination of MDA and the determination of xanthine oxidase enzyme SOD.HE staining of liver tissues pathological changes observed,using terminal deoxynucleotidyl transferase nucleotide acid -mediated deoxy-pyrimidine nucleotide biotin nick end labeling(TUNEL) detection of apoptosis,detected by immunohistochemical Bcl-2 protein expression,used spss11.5 software,measurement data with mean±standard deviation of that group compared with more than one-way ANOVA,group 22 compared with q test,P<0.05 that the difference was statistically significant.Results①serological examination:ischemic 30min,A,B,C group ALT,AST values compared,There was no significant difference(P> 0.05); reperfusion 1h,6h,24h,A group of ALT,AST with 30min of ischemia significantly higher than that,and then reached its highest point reperfusion 6h,24h reperfusion but declined slightly higher 1h of reperfusion,B group,C group after reperfusion,respectively,at each time point compared with the A group were significantly lower,the difference was significant(P<0.05,B group and C group at each time point compared with no significant difference(P>0.05);②examination of liver tissue homogenate:reperfusion 1h,6h,24h,A group of MDA with ischemic 30min significantly increased compared to,SOD significantly decreased reperfusion 6h,respectively,the highest point and lowest point,and then decrease reperfusion 24h MDA but higher 1h of reperfusion, and SOD increased;B group,C group MDA each time point compared with the A group were significantly lower,the difference was significant(P< 0.05,B group and C group at each time point SOD,respectively,compared with the A group were significantly increased,the difference was significant(P<0.05),B group and C group at each time point compared with no significant difference(P> 0.05);③Immunohistochemistry: reperfusion 1h,6h,24h,A group of apoptosis index significantly compared with the 30min ischemia increased,Bcl-2 positive index was significantly reduced reperfusion 6h,respectively,the highest point and lowest point, 24h reperfusion decreased apoptotic index but higher 1h of reperfusion, Bcl-2 positive index increased;B group,C group apoptotic index at each time point compared with the A group were significantly lower,the difference was significant(P<0.05);B group and C group at each time point index of Bcl-2 positive,respectively,with the A group significantly increased compared,there was a significant difference(P <0.05);ischemic 30min,B group of Bcl-2 positive index is much higher than its post-reperfusion time points each;ischemic 30min,C group of Bcl-2 positive index is much higher than its post-reperfusion time points each;B group and C group at each time point Bcl-2 positive index there was no significant difference(P>0.05).Conclusions①hepatic ischemia-reperfusion injury can cause significant liver damage,accompanied by lipid peroxidation and liver injury in an increase in apoptosis.②hepatic ischemia-reperfusion prior to the salidroside decoction can significantly reduce the MDA content,enhanced SOD,the protection of liver function.③Rhodiola has a strong ability of anti-ischemia and hypoxia,may be by improving the organization of the Bcl-2 protein expression and antagonism of hepatic ischemia-reperfusion injury and reduce apoptosis in the purpose to protect the liver.