Research On ACE2, TNF-α And MMP-2 Of Panax Notoginsenoside To The Left Ventricular Remodeling Of Post-myocardial Infarction

Backgroud:Radix Notoginseng is the dry root of Panax notoginseng(Burk.) F.H.Chen.It belongs to the Araliaceae family plant and is luxurious traditional Chinese herbal medicines.The modern chemistry and pharmacology learns to find that the main avtive ingredient of Radix Notoginseng is panax notoginsengsaponin(PNS),whichreaches to 12%in the content of its dry root and can inhibit thromboxane formation,extend the peripheral blood vessel,reduce blood pressure and has a notable effect in the cardiovascular and cerebrovascular diseases treatment.Respectively,in the blood system, cardiovascular system,nervous system,metabolism,as well as anti-inflammatory,anti-tumor and other aspects have a better activity.There is a relationship of RAS,ACE2,TNF-αand MMP-2 with MI-LVRM,which is we discuss about PNS on.Objective:The rats' post-myocardial infarction(MI) model was set up with ligation of left anterior descending coronary artery.Our research can observe the effect of ACE2,TNF-αand MMP-2 in rats with MI-LVRM.Methods:By ligation of left anterior descending coronary artery,building rat model of MI,after 24 hours of postoperative,the rats were randomly divided into control and experimental groups,then respectively garaged NS and the low,middle ane high dosage of PNS for four consecutive weeks.Examining the cardiac function of the rat with color doppler ultrasound,our research can investigate the protective effects of acute myocardial ischemia to PNS though the effects of PNS to biochemical indicator,myocardial morphology and hemodynamics of the rat.It also can provide experimental basis in expanding the clinic use of PNS.In the experiment,compared with the NS group:EF,FS and MV can be significantly improved by PNS in middle and high dosage group.PNS can be significantly improved pathological changes and cardiac index of left ventricular cardiac myocytes' morphology rats,the biochemistry index MDA can be significantly reduced,the activity of GSH-Px significantly enhanced,the high-dose PNS group can decrease NO.PNS can stimulus ACE2 to inhibit the expression TNF-α,inhibiting lipid peroxidation in rats.TNF-αand MMP-2 can be significantly decreased by PNS in low,middle and high dosage groups,which enhanced left ventricular systolic and diastolic function,decreased peripheral resistance,and improve the cardiac function in post MI LVRM.Results:Compared with model group,MDA is significantly lower,the activity of GSH-PX significantly enhanced(P＜0.01～0.05),the high-dose PNS group can decrease NO(P＜0.05),EF,FS and MV can be significantly improved by PNS in middle and high dosage group(P＜0.01～0.05),ACE-Ⅱcan be increased and TNF-α,MMP-2can be significantly decreased by PNS in low,middle and high dosage groups(P＜0.05).Conclusion:The result shows that,PNS can stimulus ACE2,then inhibit or reduce the expression of TNF-αand MMP-2,which inhibiting lipid peroxidation,reduce pathological injury of the mice cardiac myocytes,enhance the antioxidance,inhibit cardiac hypertrophy and improve ventricular remodeling,morphological structure of cardiac myocytes in MI-LVRM rats.