| Chinese medicine is a treasure of Chinese nation,which has a long history, with the exact efficacy,low toxicity.There is an increasing attention to the world.In the modern study framework of the Chinese medicine,the quality has been in urgent need of one of the major problems to overcome.At present,the wider application of traditional Chinese medicine quality evaluation method is known for one or two major components of the active ingredient or ingredients to be detected as a control indicator.However,the efficacy of traditional Chinese medicine to play is the result of the hole medicinal substances.The traditional Chinese medicine theory and medical practice challenges to the existing pattern of quality control,that is,any kind of determination of the active ingredients are not on their intrinsic quality.Fingerprinting of Chinese medicine is widely accepted at home and abroad to a quality evaluation model.The amount of information provided by the Fingerprinting,and to reflect the holistic medicine and ambiguity.It is an effective and feasible method to the quality control of the Chinese medicine.The research is to explore the application of this new fingerprint technology which could be used for the quality control of traditional Chinese medicine.1.Patchouli characteristics of GC-MS fingerprint "digital standards" Construction and Application(1) In the tutor group the basis of preliminary studies,combined with the literature data mining,the establishment of Shipai,Gaoyao,Hainan Agastache "the characteristics of fingerprint data."(2) In order to verify its usefulness,the paper sold a batch of 24 ingredients,three kinds of compound drugs containing patchouli analysis with Shipai,Hainan,Gaoyao patchouli "digital" information than similarity right.(3) The use of GC and GC-MS analysis of 10 batches of samples simultaneously in order to study the applicability of the GC.The results show that the medicinal product in the 24 batches,22 batches of origin can be identified,suggesting that the "digital standards" can be used for the identification of medicinal herbs;in 3 Chinese herbal compound, the Zhengqi supercritical fluid extract of Agastache rugosa in Hainan production,Zhengqi pills,capsules and soft Zhengqi Digital Hainan Huoxiang closest similarity model;in the same chromatographic conditions,patchouli essential oil from the characteristics of GC-MS fingerprint patterns can be reflected in gas chromatography,the GC-MS fingerprinting patterns in the GC with the promotion of use of the value.2.Villosum characteristics of GC-MS fingerprint "digital standards" Construction and Application(1) using GC-MS analysis of 20 batches of the major components of Amomum villosumAt the same time,since 1985,major research units of the 16 batches of Amomum villosum data mining reported in the literature.The results showed that 20 batches of Amomum villosum containα-pinene,Mainzene,β-pinene,β-myrcene,limonene,linalool,camphor,isoborneol,borneol,bornyl acetate 10 the main features of a component of the total relative content of 86.35%±5.69%,with a certain representation;the past 20 years more than 10 major domestic research units Amomum villosum are reported to contain characteristics of more than 10 ingredients,and relatively similar content. Features of this 10 component indicators,the analysis of samples in this article reported in the literature of the mean and the mean similarity of samples is 0.990(angle cosine law),which combined data processing in order to establish a broadly representative Amomum villosum characteristics of GC-MS fingerprint of the "digital" information.the digitalized characteristic fingerprint information pattern of volatile oil of Amomum villosum indicated in quality evaluate of medical material that the quality of 85%Amomum villosum was stable.The similarity result of literature data as the same as original writer.It was displayed in variety certification of medical material that only Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen is very alike with Amomum villosum and it can be distinguished between Amomum xanthioidis semen,Lout.,Alpinia chinenses,Alpinia japonica,Amomum thyrsodlum and Amomum villosum by the digitalized characteristic fingerprint information pattern.The digitalized characteristic fingerprint information pattern of Amomum villosum can be used to compare the result between different time,machines,columns and conditions. The method is reliable to evaluate quality of Amomum villosum.(3) GC applicability of studies have shown that the use of GC and GC-MS analysis of 13 separate batches of essential oil from Amomum villosum,Amomum villosum batches of the same volatile oil from the appearance of the two maps look very similar,13 samples approved Amomum villosum GC and GC-MS characteristics of indicators of the similarity in base composition of 0.994 -1.000(angle cosine law) between,GC analysis of model results and the similarity of the figures for 0.986-0.998 between the angle cosine similarity in the scope of natural fluctuations.3.Zhengqi agents fingerprint characteristics and application of Construction(1) agents Zhengqi GC-MS fingerprint mapping method using GC-MS batch of 32 different formulations,different manufacturers,different batches of samples preparation Zhengqi analysis,results showed that the samples contain limonene,purple Su-one,β-patchouli ene,β-elemene,trans-caryophyllene,α-Guaiene,gill Turcz ene,α-patchouli en-,δ-Guaiene,β-E oil alcohol, patchouli alcohol,ketone patchouli,palmitic acid,oleic acid and linoleic acid,magnolol did not identify 1,no identification of 2,and Magnolol major components,such as 18.This is the 18 major components of the peak and the relative content of the order in which they have the characteristic can be used as Zhengqi preparation and identification of the intrinsic quality of the basis for evaluation.(2) agents Zhengqi HPLC fingerprint characteristics of the construction of 29 approved the use of HPLC Zhengqi preparation of samples for analysis and the use of "similarity of chromatographic fingerprints of Chinese medicine evaluation software" of different forms,different manufacturers,different batches of Zhengqi contrast agents for similarity.The results showed that 90% of the Zhengqi agents and even better quality and stability,angle cosine similarity are greater than 0.90;the same manufacturer in different batches of high similarity between samples from different manufacturers there is a big difference in the quality of products;Zhengqi 4 pills similarity between manufacturers in between 0.744 -0.948;Zhengqi 5 capsule manufacturers in the similarity between 0.853-0.984;Zhengqi soft capsule manufacturer of two similarity to 0.939;Zhengqi water three manufacturers similarity in the 0.905 -0.938;different formulations of prescription products the same as there is a big difference,pills and capsules of similar samples to 0.880;soft capsules and liquid,tablets,pills in the similarity between 0.763-0.959.Zhengqi quality control of preparations for the study provides a reference.4.Zhengqi process of preparation of the follow-up analysis of fingerprint Zhengqi agents through various forms during the preparation of aqueous, ethanolic,finished all the steps of a comparative analysis of chromatograms, suggesting that the basic product for the characteristic peaks of the characteristic peaks of prescription medicines and the increase did not generate a new peak.(1) pill Zhengqi results showed that:①Fengfeng pill characteristics of the area of medicinal herbs in the process although in varying degrees in the lower,but the final 40 of the transfer characteristic peak rate reached 76.14 percent on average,a higher transfer rate;②has identified six characteristics of the components can be fully retained,and the transfer rate of 69.13-86.Between 93%;③Pill Zhengqi components market to retain the results of the analysis also shows that 80%of Zhengqi medicine pills to retain most of the characteristic peaks with Zhengqi scattered than the results of the similarity is greater than 0.90;To sum up,the preparation of pills Zhengqi more reasonable,able to retain most of the characteristics of composition;pills from volatile oil GC-MS analysis,the pills in the retention of volatile oil components are not too satisfactory results,suggesting that pill Preparation process should be improved methods of volatile oil extraction.(2) Zhengqi Capsule capsule and prescription pills are the same,the results showed that:①the average capsule finished pills low transfer rate i0.28%;②has identified six characteristics of the components of which only the four, the transfer rate of 56.75-74.94%;③Zhengqi capsuleingredients market to retain the results of the analysis also shows that the composition of capsule Zhengqi reservations worse than the pill;from the capsules of volatile oil GC-MS fingerprint analysis,the capsules of volatile oil components in the retention of results than the pill,and prompted the preparation of capsules for further improvement.(3) Zhengqi soft capsule,water,three belong to the same prescription tablet and is similar to the preparation process,results showed that:①This form three kinds of agents results finished transfer rate close to 70.74%, respectively,67.50%,70.35%;②market Zhengqi soft capsule,water,tablets ingredients to retain the results of the analysis also shows that these three agents are able to better retain the characteristics of prescription medicines in the composition,suggesting that these three forms of the preparation process more reasonable;③from the volatile oil GC-MS fingerprint analysis, the soft capsule composition of the volatile oil to retain the most complete, tablets of the content of volatile components as the extension of storage time reduced;Zhengqi in water because of its accessories containing ethanol,so fewer components detected.Zhengqi prompted Zhengqi water and the preparation of films are to be further improved.This experiment Zhengqi agents through the establishment of medicinal materials,semi-finished and finished products fingerprint for the preparation of the prescription medicines Zhengqi extraction methods and the extraction rate to provide some data,the process for preparation Zhengqi study provides some reference.
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