Study On The Cerebral Protective Effects Of Recombinant Human Erythropoietin On Hypoxic-ischemic Brain Damage In Neonatal Rats

ObjectiveTo explore the influence of recombinant human erythropoietin(RhEPO) on MDA,SOD,NO and neuronal apoptosis in the brain tissue of hypoxic-ischemic brain damage(HIBD) in neonatal rats. Elucidate protective effect of RhEPO to HIBD in neonatal rats.Methods101 postnatal seven- days-old Wister rats were divided into three groups,Sham operation group(n = 25),control(normal saline NS) group(n = 38),experiment(RhEPO)group(n = 38). Control and experiment groups were ligated right common carotid artery and laid back to their former condition for 3 hours.Mixture of oxygen with volume fraction of 0.08 and nitrogen with volume fraction of 0.92 was sucked for 2 hours.Every rat of control group was injected intraperitoneally into 0.5ml NS after HIBD.But the rats of experiment was injected RhEPO(4000 U/kg) 0.5ml.The rats of Sham operation group were only dissociated of the right common carotid artery without ligation.SOD activity and MDA,NO level were measured at 2,12,24,48,72,96h after the treatment. The brain tissue slices at 24h was detected by HE and Tunel staining,the structure and the numbers of cerebral apoptosis were detected under the light microscope.ResultsSOD of control group decreased markedly compared with Sham operation group,in the 2-96 hours(P<0.01),But MDA increased,in the 2-72 hours(P<0.01).NO markedly increased at the 2 hours(P<0.05),in the 12-96 hours(P<0.01).SOD of experiment group increased markedly compared with control group,in the 12-96 h(P<0.01),but MDA decreased,in the 24-72 hours(P<0.01),NO decreased markedly in the 24-96 hours(P<0.01).By HE staining,the typical apoptosis cells in the hippocampal CA1 region of the observation group decreased compared with the control group.ConclusionRhEPO has cerebral protective effects that gets by increasing SOD and decreasing MDA,NO in the brain tissue and restraining neuronal apoptosis of HIBD in neonatal rats...