The Study On The Protective Role Of Melatonin Against Oxidative Injury Of Doxorubicin In Rats

Objective To investigate the injury of DX during chemotherapy and protective role of MT to liver, Kidney, heart and brain in the rats.Methods fifty male Wistar rats, were randomly divided into following groups and gave different treatments respectively ,①control, saline solution were administered into the tail vein, only once;②MT, a dose of MT(5mg/kg ) were administered subcutaneously;③DX, a dose of DX (25mg/kg ) were injected in the tail vein, only once;④MD, MT were administered an hour first before injection of DX, 7D successively;⑤DM, DX were administered an hour first before injection of MT, 7D succesively.after one week, animals were sacrified .the content of MDA and the activities of GSH in the liver, kidney , brain and heart were examined; the degrees of creatinine(Cr), blood urea nitrogen (BUN),serum-albuminn(Alp), glutamic oxalacetic transaminase(AST), isoenzymes of creatine kinase (CK-MB)and Lactic acid dehydrogenase (LDH), total-protei(Tp) in blood were also tested.Result 1.Dealt with by the DX rats, there make them apathetic, dull hair, loss of appetite, diarrhea and other symptoms.Reported in the literature of the DX and toxicity symptoms similar to [1].Detected in the serum of the project, TP,Alb decreased, other items AST, BUN, Cr, LDH,CK-MB levels were significantly higher, P <0.05.Detection in the organization, liver, kidney, heart, brain tissue was significantly increased MDA content, GSH activity was significantly lower under, P <0.05.2.DM group serological changes were improved, TP,Alb increased, AST, BUN, Cr, LDH,CK-MB decreased, though it has yet to return to the control group level, organizations also decreased MDA content, GSH activity picked up.3.MD group, liver and kidney MDA content in no significant change; liver, kidney, brain tissue no significant changes in GSH activity.4.MT5mg/kg dose not cause harm to the organization's performance. Conclusion 1.DX conclusions can be caused by chemotherapy in vivo liver, kidney, heart, brain and other organs of the oxidative damage, resulting in lipid peroxidation product of MDA content increased significantly, lower in liver, kidney, heart, brain tissue GSH antioxidant enzyme activity , serum AST, BUN,CR, LDH, CK-MB and a significant increase in Alb,TP will be an obvious decline.2.In the DM group, add the MT can play a significant protective effect, reducing lipid peroxidation product of MDA content, improve liver, kidney, heart, brain tissue GSH antioxidant enzyme activity, significant changes in serum biochemical indicators.The mechanism may be related, directly or indirectly, free radical scavenging, and enhance antioxidant enzyme activity.3.MD group, MT failed to significantly change the liver and kidney MDA content; liver, kidney, brain tissue was significantly increased GSH activity.4.High-dose MT (5mg/kg), there is no damage to the tissues and organs function, is safe.