| Objective: Peripheral nerve compression are common, multiple injury. This nerve injury can cause neurological dysfunction, serious impact on quality of life of patients. Exquisite microsurgical technique for the compression of neural regeneration and repair to provide a good foundation. However, after nerve compression, how it would be protected, to minimize the secondary damage, quickly restore its conduction function is still not yet solved。In recent years, domestic and foreign to do a great deal of relevant research and found that some of the pressure of nerve regeneration and repair have to promote the role of biologically active substances and related drugs (such as: neurotrophic factor, Extract from Rabbit Skin lnflamed by Vaccinia Virus for lnjection), and after becoming the compression field of nerve regeneration and repair another hot spot.Extract from Rabbit Skin lnflamed by Vaccinia Virus for lnjection (ERSVV) is a new type of China-made biological agents, are sensitized by the cowpox virus vaccine in rabbits after the purification of delicate skin tissue fluid with nerve repair, analgesic, immune regulatory role. It has intramuscular injection, intravenous injection, intraperitoneal injection, nerves around the administration, such as subarachnoid administration route of administration. However, injected directly into the damaged ERSVV perineural and intramuscular injection pressure on the card after nerve regeneration and repair has not been reported there is no difference. SD rats used in this experiment, in the light of Bennett and Xie designed compression model of chronic sciatic nerve (chronic constriction nerve injury, CCI), to explore two different ERSVV route of administration pressure on the card after nerve regeneration and repair. Clinical treatment is designed to provide the basis of peripheral nerve injury.Methods: 80 male SD rats, weight 280±20 grams, were randomly divided into 4 groups of 20: A group for the sham operation group (normal control group), B group, model group (the pressure of the control group), C group intramuscular ERSVV, D group was given perineural ERSVV. After the success of narcotic, A group show only the right side of the sciatic nerve rather than compression, that is closed incision; B, C, D groups were prepared in the right thigh CCI model, and neural tube at the side tube. Every day transcatheter injection water for injection 0.2ml, to prevent catheter obstruction. Rats 14 days after the strongest behavioral responses, but also bowel, based on endoscopic observation after the nerve edema, axonal degeneration and reduction of the number of myelin disintegration (Guilband reports required of the model set up 14 days) to prove that CCI Model Preparation success. Group B continued to 14 days after the transcatheter injection 0.2ml water for injection until subjects. C Group B Group based on the intramuscular ERSVV0.2ml, and D group will be re-ERSVV 0.2ml water for injection until subjects。CCI separately in four groups after 14 days, 21 days, 28 days measured in rat sciatic nerve function index (Sciatic functional index, SFI). And at random from each group 6 rats, the sciatic nerve exposed under anesthesia to observe general changes in nerve, nerve conduction velocity measurements (nerve conduction velocity, NCV) and amplitude (Amplitude, APT) and drawn in light and electron microscopy Ultrastructure of sciatic nerve under observation for design analysis and calculation the number of axons and myelin sheath thickness, immunohistochemical detection of NGF (Nerve growth factor, NGF).Results: 1. Gross observation: CCI rats after general condition, body weight not significantly reduced, hair full of luster, feeding normal drinking water. Limb to reduce the activity, weakness, claws extended limited, there licking feet, grooming, scratching and other symptoms, and gradually increase. Beginning after administration, the rats gradually restore limb activity, gradually reduce the symptoms. At 14 days after operation, A group of nerve smooth surface, with the surrounding tissue were no adhesions. B, C, D groups of neurons marked hyperemia, edema; compression paragraph epineurium color than red. Terms of a formulation in continuous time with the drug application, at 21,28 days after the first B group hyperemia, edema is still heavier, C, D groups hyperemia, edema significantly reduced the pressure of paragraph epineurium of the white color gradually . And the D group and the color epineurial edema than C group.2. Sciatic function index (SFI): with the A group, B, C, D Three days SFI Group 21,28 significantly reduced (P <0.05). And the B group, C, D were the first days 21,28 SFI was significantly increased (P <0.05), while the D group was significantly higher than C group (P <0.05). C, D, respectively, the two groups 28 days and 21 days, 14 days for comparison group was significantly higher (P <0.05; P <0.01).3. Neuroelectrophysiological Analysis: A group NCV; AMP at various test points no significant change. C, D group than the B group 21,28 days and lower than the A group (P <0.05), D group was higher than C group (P <0.05). Group Comparison: B group increased obvious there was no significant difference (P) 0.05); C, D groups of 28 days was superior to 21 days (P <0.05), more obvious than 14 days (P <0.01 ).4. Axon count and myelin thickness: with the A group, at different time points B, C, D group was significantly smaller (P <0.05). 21,28 at the first timing, C, D group were higher than those in B group (P <0.05), D group was higher than C group (P <0.05), and C, D group, 28 days were higher than No. 14 in this group, 21 days the value of ((P <0.05). However, group B no significant increase was not obvious (P> 0.05)5. Histopathological observation, light microscopy: A group of nerve fibers were arranged regularly, myelin integrity. 14 days in section B, C, D showed similar neural substantial fracture, nerve edema severe loss of myelin and nerve axons reduced density. B Group of 28 days, there is a small nerve growth but no myelin, C group can see the myelin sheath thickening, an increase in quantity, D group at 28 days and there is small there is a thin sheath of nerve axons germination. Electron microscope: A section 14 days nerve myelin, nerve cells and basement membrane integrity of the structure, lamellar myelin clearly visible. B, C, D group significantly myelin edema, mitochondria and microfilaments microtubules significantly reduced, some myelin myelinated nerve hyperplasia irregularly shaped plate edema. B Group of 28 days some axons fracture, spinal fusion disappear, mitochondria cavitation, lamellar structural damage, tubulin microfilament reduced lesser injury. C group disappeared most spinal fusion, tubulin microfilaments can be seen, a lesser injury, myelin thickening, lamellar change the rules, but the D group has the number of myelinated nerve fiber, axon diameter, myelin thickness, axon within mitochondria, microfilaments, microtubules, etc. Quantity is greater than C group.6. Immunohistochemistry: A group of non-expression or a small amount of NGF expression. And B, C, D group NGF expression in the degeneration and regeneration of axonal nerve fibers and Schwann cells (SC) are visible expression of the membrane, with an area of large and small, Boundary less clear, showing diffuse brown coloring. Image analysis results showed that: NGF the average gray Index: B, C, D group at a gradual increase in 21,28 days; C, D group than the B group, D group than the C group (P <0.05). And C, D Group at its 28 days and 14,21 days in this group than increased (P <0.05).Conclusion: 1 ERSVV conducive to the regeneration of nerve injury and repair。2 ERSVV nerve damage around the administration of nerve regeneration and repair better than intramuscular.
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