The Establishment Of Using Liquid Chip Technology To Quantify Prostate Specific Antigen (PSA) In Human Serum And Urine And Its Use In The Diagnosis Of Prostate Cancer

Objective To establish the response mode by the use of liquid chip technology to quantificationally detect the serum prostate specific antigen (sPSA) and urinary prostate specific antigen (uPSA) and evaluate this method. The present study was to apply this mode to diagnose prostate cancer(PCa) and judge of the effect of sPSA, uPSA and ratio of urinary and serum prostate specific antigen (u/sPSA ratio) in the diagnosis of prostate cancer(PCa).Methods By using the Liquid chip technology, we were to detect the PSA level in the serums of 50 patients with benign prostatic hyperplasia(BPH) and then evaluated the indexes of this method, such as linear detection range, minimum detectability, precision and so on. Whereafter, we would perform correlation analysis for the results between the Liquid chip technology and chemiluminescence immunoassay (CLIA). Besides, the Liquid chip technology was also applied to another group of patients,22 diagnosed PCa patients and 30 cases of the aforementioned 50 BPH patients, to detect their sPSA level, uPSA level and calculate the u/sPSA ratio, respectively. By comparing the three indexes of sPSA level, uPSA level and u/sPSA ratio between the two groups, we were to evaluate the value of the above-mentioned three indexes in the diagnosis of PCa.Results According to the PSA standard curve after detection, we could get some index values, such as linear range (0.022-129.6ng/ml), the minimum detectability (4.8pg/ml), and the intra- and inter- assay coefficient of variation(CV) were 2.18%～2.28% and 1.61%～4.18% respectively. Liquid chip method and CLIA were used to quantify PSA in human serum. It was concluded that there was no significant significance between the two methods (P>0.05),and they had a good correlation, r=0.9684 (p<0.001,n=50). The study indicated that the sPSA levels for both of PCa group and BPH group were more than 10.0ng/ml, which was no statistical significance. The uPSA level of PCa group is higher than that of BPH group, which was also in no statistical significance. But the u/sPSA ratio of PCa group was rather higher than that of BPH group (p<0.05), which has statistical significance.Conclusions Liquid chip technology is endowed the advantages of wide linear range, high sensibility, good repeatability, less samples and time-saving, which also has great potential clinical applications. The u/sPSA ratio can be as a method to differentiate between Pca and BPH. Even if the method doesn't work for the distinction, it still could have the screening value. The detection of u/sPSA ratio can usefully decrease the rate of unnecessary prostate biopsy. Meanwhile, the detection is quite simple and easy to perform and is fit to popularize in clinic.