| Objective1. To invent a package system for transportation of cornea preserved both in intermediate term medium and in freezing glycerol.2. To study the temperature-time graphs and the maintaining time of available temperature (0～4℃) while corneas are preserved in the system with intermediate term medium. To discuss the security and the feasibility of cornea transportation in the package system.3. To study the temperature-time graphs and the maintaining time of available temperature (≤-20℃) while the corneas are preserved in the system with freezing glycerol. To discuss the security and the feasibility of cornea transportation in the package system.4. To perform the PKP operation with the ITMS (intermediate term medium storage) corneas that have undergone imitated transportation in the package system. To study the impact on the implant and CEC viability after transportation.5. To perform the PKP operation with the LTFGS (Long term freezing-glycerol storage) corneas that have undergone imitated transportation in the package system. To study the impact on the implant after transportation.Methods1. In normal temperature condition, lay ice box and ITMS (intermediate term medium storage) bottle together in the system, which are both 4℃, with sponge stuffing the spare room. Divide into 4 groups by the cubage of the intermediate term medium and record the temperature curve both in-bottle and ex-bottle. Observe the maintaining time of the temperature between zero and 4℃, and analyze among the groups. Compare the cornea position before and after imitated transportation in the package system.2. In normal temperature condition, lay -21℃ice box and -25℃freezing-glycerol bottle together in the system, with sponge stuffing the spare room. Divide into 4 groups by the cubage of the glycerol, and record the temperature curve both in-bottle and ex-bottle. Observe the maintaining time of the temperature under -20℃, and analyze among the groups.3. 8 corneas were distributed into the experimental group which have been preserved with intermediate term medium for 7 days. To perform an imitated transportation by vibration according to a certain frequency and time. Then take the PKP operation with these corneas, and stain the remaining cornea with trypan blue and alizarin red to examine. And the other 8 corneas were distributed into the control group, also were preserved with intermediate term medium for 7 days. Take the PKP operation and examine the remaining cornea ring without transportation. To examine all the albino rabbits after the PKP by general observing, slit-lamp examination and A-scan cornea thickness measuring. Kill all the rabbits 30 days after PKP, and examine the implant CEC with trypan blue and alizarin red staining.4. 8 corneas were distributed into the experimental group which have been preserved with freezing glycerol for 4 months. To perform an imitated transportation by vibration according to a certain frequency and time. Then take the PKP operation with these corneas. And the other 8 corneas were distributed into the control group, also were preserved with freezing glycerol for 4 months. Take the PKP without transportation. To examine all the albino rabbits after the PKP by general observing, slit-lamp examination and A-scan cornea thickness measuring. Kill all the rabbits 30 days after PKP, and examine the implant CEC with trypan blue and alizarin red staining.Results1. In normal temperature condition and in a definite range of medium cubage, the package system can maintain the temperature between zero and 4℃for about 20h, which is much longer than ice-bucket. The differences between experimental groups and control group are significant.2. In normal temperature condition,in experimental groups, the geometric means of glycerol temperature in 24 hours are all under -20℃,but in the control group, the temperature of 24 hours are higher than -17℃entirely. The maintaining time of temperature under -20℃is more than 17 hours in experimental groups while the volume of glycerol is less than 300ml. The differences between experimental groups and control group are significant.3. The data between experimental group and control group have no statistical difference, such as the CEC staining examination of cornea ring, the cornea thickness after PKP, the cornea edema regression time, the implant CEC staining 30 days after PKP.4. The data between experimental group and control group have no statistical difference, such as the cornea thickness after PKP, the cornea edema regression time, the implant CEC staining 30 days after PKP.Conclusion1.In normal temperature condition, with 4℃ice-box the package system can control the temperature between zero and 4℃effectively, and this temperature range is necessary for preserving cornea with intermediate term medium. The system can maintain this temperature range for more than 20h, and the time is enough for transportation to most of areas in the world. The package system also has the characteristics of vibration resisting and cornea immobility. It is secure for intermediate term cornea storage and feasible for long-distance transportation.2. In normal temperature condition, with -20℃ice-box the package system can control the temperature under -20℃effectively, which is necessary to preserve cornea with freezing glycerol. The system can keep this temperature range for more than 20h, and the time is enough for long-distance transportation to most of areas in the world by proper vehicles currently.3. There was no significant impact on the cornea viability after transportation by the ITMS cornea transportation system. The results of PKP were reliable. The system could work as an important part of field eye bank(FEB), could meet the demand of the transportation of ITMS cornea either in peacetime or in wartime.4. The results of PKP were reliable after transportation by the freezing glycerol preserved cornea transportation system. The system could work as an important part of FEB, could meet the demand of the transportation of LTFGS cornea either in peace time or in war time. |
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