Effects Of SiRNA Targeting VEGF Gene On VEGF Expression And Cell Proliferation In HeLa Cell Lines

Angiogenesis is the necessary process of tumor occurrence and development, it is closely related to tumor growth and metastasis. A number of growth factors have been identified as potential positive regulators of angiogenesis. Among them, vascular endothelial cell growth factor (VEGF) is known to be one of the most important inducers of angiogenesis. VEGF is not olny can involve in tumor angiogenesis directly or indirectly to promote tumor growth, but also can stimulate the proliferation of tumor cells in the form of autocrine. Therefore, VEGF has an extremely important position in the occurrence, develpoment and prognosis of malignant tumors. Different studies demonstrated that increased levels of VEGF expression have been found in most human tumors, such as cervical cancer, suggesting that it might be an attractive target for therapeutic intervention aiming at preventing tumors from recruiting the blood supply that they need to survive. RNA-mediated interference is a conserved gene silencing mechanism that recognizes dsRNA as a signal to trigger the sequence-specific degradation of homologous mRNA and post-transcriptional gene silencing as a result. RNAi technology provides new ideas for highly specific gene silencing therapy.In this study, we selected cervical cancer cell line HeLa cells which has a high expression of VEGF as subjects. After transfacting VEGF gene-targeted small interfering RNA vectors into HeLa cells, we established stable expression of the VEGF siRNA cell lines. The purpose of this experiment is to investigate the effects of small interfering RNA targeting vascular endothelial growth factor (VEGF) on VEGF expression and cell proliferation of cervical carcinoma HeLa cells.The results suggested that siRNA can effectively knock down the expression of VEGF and inhibit the cell proliferation in cervical carcinoma cells. It shows that VEGF play a role in the occurrence and development of cervical carcinoma. Inhibiting the expression of VEGF is expected to become an effective means of treating cervical cancer.