Molecular Beacon Probe Melting Curves Analysis For Rapid Screening Of Ethambutol Drug Resistance In Mycobacterium Tuberculosis Clinical Isolates

We have developed a modified molecular beacon melting curve analysis for rapid screening of Ethambutol(EMB) drug resistance and multi-drug MDR) resistance tuberculosis.A total of 107 isolates including 84 culture samples and 23 sputum were provided by Xiamen CDC,Shenzhen Center for Chronic disease Control and Peking University of Medical College.Our system was able to detect a single copy of M. tuberculosis,screen out wild type from mutant type and even screen different bases mismatch.All the wild types and mutant types were confirmed wild types and mutant types by sequencing.The system could only detect M.tuberculosis giving a specificity of 100%.In order to reduce MDR in M.tuberculosis,we recommend our molecular method detection to rapidly adjust the therapy in drug resistance patients.