The Distribution Of Gentamicin In The Hair Cells Of The Inner Ear Following Gentamicin Application Through Endolymph Or Perilymph Approach

Purpose The distribution of gentamicin in the inner ear hair cells of guinea pigs after endolymph or perilymph application was investigated using a fluorescent probe gentamicin-Texas red conjunction(GTTR).Methods Adult guinea pigs(n=10) were administered GTTR through the endolymph and perilymph approach by the endolymphatic sac and intact round window membrane.Animals survived for 3 days.Laser confocal microscopy was used to observe the distribution of GTTR in the cochlear and vestibular hair cells.Resultsâ‘ When GTTR was administrated through the endolymphatic sac, stong GTTR labeling was observed in the hair cells of saccule while it was less in the hair cells of utricle and 3 ampullae.Very few GTTR labeling was observed in the basilar membrane of the cochlear.â‘¡When GTTR was administrated through the intact round window membrane,GTTR labeling was observed in the outer hair cells of the basilar membrane and hair cells of vestibular organs.Conclusion GTTR had a less accumulation in the outer hair cells of the basilar membrane through endolymphatic sac administration than that through round window administration.The results may indicate that endolymphatic sac administrated gentamin is able to cure intractable vertigo of Meniere disease meanwhile decreasing the cochlear toxicity.