A Study Of The Relationship Between Autoimmune And Secondary Brain Damage After Traumatic Brain Injury

Objective:To study the changes of the Antibrain-Antibody(ABAb) in patients with the Traumatic brain injury.Antibrain-Antibody were injected to the cortex of rabbits,study the damage of brain tissue caused by Antibrain-Antibody.To establish a animal model of severe lateral fluid percussion brain injury in rabbits. Immunosuppression therapy was employed on it,demonstrate the cerebral protective effects of immunosuppression therapy after the Craniocerebral Trauma. Methods:A three-phase-work was designed,at first,The serum concentrations of ABAb were measured with ELISA in 49 cases of Traumatic brain injury an 46 cases of healthful adults.All patients with the Traumatic brain injury were phlebotomized blood 4ml through the ulnar vein at 3d,7d,2w,3w,lm,2m,6m,9m after injury,the blood serum must be centrifuged used centrifugal machine,and then conserved with—20℃.Blood serum specimen were tested by ELISA to observe the dynamic change of Antibrain-Antibody(ABAb).Sceond,To separate rabbits into ABAb group(n=8),immunosuppression therapy group(n=8),NS group(n=8).ABAb or NS were injected to the cortex of rabbits.Expression of Glu were examined by means of immunohistochemistry in the cerebral cortex of rabbits.apoptosis of the neurons was determined with TUNEL staining.Third,To separate rabbits into TBI group(n=8),sham operation group(n=8),immunosuppression therapy group(n=8). Establish a animal model of severe lateral fluid percussion brain injury(LFPI) in rabbits.immunosuppression therapy group was feeded CsA(50mg/2d) by the tummy duct.All group rabbits were phlebotomized blood 4ml through the ear fringe vein at 2h before injury,3d,7d,14d,21d,28d,35d.42d,49d after injury.Blood serum specimen were tested by ELISA to observe the dynamic change of Antibrain-Antibody(ABAb), Expression of Glu were examined by means of immunohistochemistry in the cerebral cortex of rabbits.apoptosis of the neurons was determined with TUNEL. Results:A few ABAb has been founded in the blood serum of health;After TBI,The concentration of serum ABAb was significantly higher in the patients with acute craniocerebral injury than that in the healthful adults(P < 0.05).1 month after the ABAb injection,comparing with NS group,neuronal apoptosis and Expression of Glu in cortex significantly increased in ABAb groups.There was statistically significant difference between ABAb and NS group and immunosuppression therapy group(P < 0.05).After LFPI,TBI group had its peak value of the ABAb,The peak value of immunosuppression therapy group lower than TBI group,while the sham operation group had no significant change.Comparing with TBI group,neuronal apoptosis and Expression of Glu in cortex significantly decreased in immunosuppression therapy group.There was statistically significant difference between TBI and immunosuppression therapy group and sham operation group(P< 0.05).Conclusions:1.There was a few ABAb in the blood serum of healthful adults.2.After brain trauma,the concentration of ABAb was higher than nature;3.After brain trauma,the increased ABAb will be likely to induce the neuronal apoptosis and increase positive of Glu neuron,,so that we said brain trauma could result in autoimmune diseases.The autoimmune maybe is a cause of secondary brain damage.4.After brain trauma,the concentration of ABAb in the blood serum could be down by immunosuppression therapy,the effect of attack self brain tissue would be alleviated consequently;5.We considered,immunosuppression therapy will become a new therapy react on brain trauma.