Diagnostic Values Of DNA-aneuploidy And C-12 Analysis For Benign And Malignant Ascites

Background and Objective: Ascites is a common clinical symptom, which results from complicated causes, often bringing difficulties to clinical differential diagnosis. Although the traditional cytological diagnosis is great for the specificity, its sensitivity for the malignant ascites is only 50%-60%. Flow cytometry and tumor-marker examining are the important methods to diagnose malignant tumor in recent years. The former finds out cell chromosome abnormalities and cell proliferative activity through measuring DNA ploidy; and the latter reflects the existence of tumor by detecting multi-specific tumor markers at a time. Therefore this paper will discuss the value of differential diagnosis for the benign and malignant ascites by using the FCM to analyze ascitic cells'DNA ploidy and the mulit-tumor marker protein chip (C12) to detect 12 kinds of the most common tumor markers.Methods; Flow cytometry(FCM) was applied to analyze ascites cells DNA ploidy from 68 identified ascties patients(31 for benign ascites, 37 for malignant ascites), which takes finding heteroploidy in the cells as masculine; meanwhile 12 kinds of common tumor markers were detected with multi-tumor marker protein chip(C12). We made T test to measurement material and chi-square test to counting material, and finished statistics analysis with the statistics software SPSS 16.0.Results: 1. The DNA index from the malignant ascites group was apparently higher than that from the benign ascites group. The sensitivity, specificity and accuracy of malignant ascites diagnosed through DNA ploidy were 72.97%, 93.45% and 82.35% respectively. 2. The contents of CEA, Ferritin,CA252,CA153,CA199 and NSE from the malignant ascites group was higher than that from the benign ascites group. The sensitivity, specificity and accuracy of malignant ascites diagnosed through the mulit-tumor marker protein chip (C12) were 94.59%, 54.84% and 76.47% respectively.3. We evaluated the differential diagnosis value of the combination of heteroploidy and protein chip tumor marker C-12, and found the combination shows a sensitivity of 94.59% and a specificity of 96.77% for malignant ascites.Conclusion: DNA ploidy analysis and protein chip tumor marker C-12 detecting are valuable biological indexes to differentiate malignant ascites from the benign. And their combination can improve the sensitivity and specificity in differential diagnosis of malignant ascites from the benign, and can also improve the diagnosis efficiency, apt for clinical application.