| Objective: To observe the dynamic change of the density of blood plasma S-100βprotein and neuron specific enolase (NSE), and the influence of inhaling different density sevoflurane to its content during cardiopulmo- sevoflurane pretreatment.Methods: Forty ASAⅡorⅢpatients aged 45-65 years old undergo- ing heart surgery under general anesthesia were randomly divided into four groups with 10 cases each: control group (no any gas anaesthesia medicine), S1 group (the density of sevoflurane:0.5MAC), S2 group (the density of sevoflurane:1.0MAC), S3 group (the density of sevoflurane:1.5MAC). Jugular vein blood was drawn following 6 time points: before CPB (Tl), 30min after the beginning of CPB (T2), immediately after the end of CPB (T3), lh after the end of CPB (T4), 6h after the end of CPB (T5) and 24h after the end of CPB (T6). The density of blood plasma S-100βprotein and NSE protein was detected by enzyme-linked immunosorbent assay. MMSE score (Mini-Mental State Examination) were determinated before and 24h after the heart surgery operation. Spss13.0 software was used for statistical analyses. Data were presented as the mean±standard error of the mean (SEM). Statistical significance of difference between means was evaluated by t-test and P values less than 0.05 were considered statistical significance.Results: Four groups of MMSE scores after operation were all less than those before operation, and the difference had statidtics significance (P<0.05); The scores of S2 and S3 group were significantly less than those of control group, The scores of S2 and S3 were significantly less than those of S1, and the difference had statidtics significance (P<0.05). The density of blood plasma S-100βprotein and NSE of four groups were in the normal range before CPB, and the difference hadn't statidtics significance (P>0.05). At 30min after the beginning of CPB, compared with the control group, the density of blood plasma S-100βand the NSE protein of each group increased, and the level of them increased to the peak immediately after the end of CPB. At 30min after the beginning of CPB, the density of blood plasma S-100βand the NSE was less than that of control group, and the difference had statidtics significance (P<0.05). Immediately after the end of CPB, 1 hour and 6 hour after the end of CPB, the density of blood plasma S-100βof S2 and S3 group was less than that of the control group, and the difference had statidtics significance (P<0.05); at 24 hour after the end of CPB, compared with the control of group, the density of blood plasma NSE of S2 and S3 group was decreased, and the difference had statidtics significance (P<0.05) and there was significantly difference in the density of blood plasma S-100βbetween the S2 and S3 group and the control group (P<0.01).Conclusion:1.The decrease of S-100βprotein and NSE density indicates that sevoflurane has the cerebral protective function for the CPB patients.2.The change of MMSE score indicates that sevoflurane could promote the cognition function of patients after CPB.3.Sevoflurane has protective effect on cerebral function in a concentr- ation dependent manner.
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