Studies On The Influence Of Different Extractions Of ASP On Synthesis Of APIC

Angelica sinensis polysaccharide iron complex(APIC), a macromolecular complex synthesized by neutralization of Angelica sinensis polysaccharide (ASP) and ferric chloride solution, is a new iron supplement with dual hematonic effects. For a highly simplified preparation of APIC that has steady physical and chemical properties and is suitable for clinical use,this paper study on the different extractions of ASP and the influence of different extractions of ASP on synthesis of APIC.Part one Studies on the different extractions of ASPThis part of experiment includes four extraction methods of ASP that are water extraction and alcohol precipitation method, acid extraction and alcohol precipitation method, alkaline extraction and alcohol precipitation method ultrasonic extraction and alcohol precipitation method. In the water extraction and alcohol precipitation method, we selected the optimum water extracting condition by orthogonal text which is: soaking 60min. extracting 120min, and 10 times solvent volume. And also we selected the optimum alcohol precipitation condition by orthogonal text which is: the density of extract is 1:1.8 and alcohol concentration is 65%with 95% alcohol. In the ultrasonic extraction and alcohol precipitation method, we selected extracting 60min.In the acid extraction and alcohol precipitation method, we selected 0.3mol.L^-1 acid concentration. In the alkaline extraction and alcohol precipitation method, we selected 0.5mol.L^-1 alkaline concentration.Comparative studies physicochemical properties of ASP with four different extraction methods were carried out. The results show that total sugar content in ASP is (15.33±6.21)g and the protein content is (33.29±3.94)%,we found that the alkaline extraction and alcohol precipitation method isn't suitable for the ASP extraction. Analysed of flourier transform infrared spectroscopy(FTIR) and UV spectra , the results show that ASP with four different extraction methods have the characterized absorbing peak of polysaccharides; The chromatograms of ASP with four different extraction methods were examined by G-50, the results show that ASP with four different extraction methods consists of two fractions but the proportion is different. Part two Studies on the influence of different extractions of ASP on synthesis of APICAPIC were synthesized by neutralization of ASP that was obtained by three extraction methods. Taking APIC_标for reference, comparative study on physicochemical properties of the three APICThe iron content was determined with iodometry. The total sugar content was determined with phenol-sulfuric acid method. The stability was tested with hydrolytic experiments and TG. The chromatograms of APIC were examined by Sephacryl gel chromatography. Analyses flourier transform infrared spectroscopy (FTIR) and UV spectra of APIC.The result indicates that the iron content of APIC was (29.08±2.06)%,the total sugar content of APIC was(11.57±0.73)%.Our studies indicate that the complex is stable at physiological pH from 6to 12, without precipitation and hydroxylation. Thermal gravimetric analysis exhibits when temperature 600℃40% mass loss. Our studies indicate that when APIC is eluted by gel chromatography, two fractions can be obtained, but the proportion is different.From all the results of our studies described above, our conclusion is that the influence of different extractions of ASP on Synthesis of APIC is no significant in the aspects of the iron content, the total sugar content, the stability and the FTIR and UV spectra. From the view of the simple, economical extraction, water extraction and alcohol precipitation method of preparing for ASP to synthesis APIC is to optimum.