The Research Of Reconstituting Streptococcus Gordonii Anti-Tuberculosis Mucosa Vaccine

Objective:To construct expressing vector carrying esat6 gene and express this protein in Streptococcus gordonii GP251.Methods: ESAT6 gene was amplified by PCR with specific primer from genome of Mycobacterium tuberculosis (MTB)H37Rv. ESAT6 was cloned to vector PSMB104 and expressed in Streptococcus gordonii GP251. The expression of esat6 protein was detected by Tricine-SDS-PAGE and Western-blot, ELISA technique was also used to detect its secretory volume.Results:It was confirmed that ESAT6 gene was cloned into expressing vector successfully, and a 10KD protein was secreted by Streptococcus gordonii GP251, this protein has a good immunogenicity.Conclusion:The expression vector of ESAT6 gene was constructed, and ESAT6 protein was expressed in Streptococcus gordonii1 GP251 successfully.It will provid some reference for the research of reconstituting anti-tuberculosis mucosa vaccine...