The Expression Of Basic Fibroblast Growth Factor Mediated By Microbubbles And Its Effect Of Angiogenesis In Ischemic Myocardium

Objective:To investigate expression of bFGF gene and its effect of angiogenesis in ischemic myocardium by ultrasound-mediated microbubble destruction,provide an experiment basis for the therapy of ischemic myocardial disease by ultrasound-mediated microbubble which carried vascular growth factor gene.Methods:Forty-six rats were randomly assigned into the control group, the gene group and the ultrasound mediated gene group.Models of acute myocardial infarction moderl were made by ligation of the left anterior descending coronary artery.A total volume of 50μl Microbubbles attached rh-bFGF(2μg/μl) were administered into the tissue by intramyocardial injection with ultrasound exposure in the ultrasound mediated gene group,while in the gene group,all animals were only treated with gene injection.Rats in the control group were infused with saline.The fluorescence intensity and expression of bFGF mRNA were decteded at 4 weeks after treatment.FactorⅧimmunohistochemistry were used to detected the angiogenesis.Results:1.The expression of gene:After 4 weeks,all animals with gene therapy can detect red fluorescence,The fluorescence intensity in the ultrasound mediated gene group was(112.67±3.61),while in the gene group it was(53.73±4.09).The expression of RFP was higher in the ultrasound mediated gene group than gene group(P＜0.05).The bFGF mRNA expression was higher in the ultrasound mediated gene group (1.247±0.323)than alone gene group(0.771±0.102)or the control group (0.473±0.153).There was significant difference in the groups(P＜0.05).2. FactorⅧimmunohistochemistry demonstrated that the capillary intensity was higher in the ultrasound mediated gene group(188.63±15.31)than the alone gene group(141.13±17.65)or the control group(75.58±6.59).Compared with gene group,ultrasound mediated gene could facilitate the angiogenesis(P＜0.05).Conclusions:Ultrasound-mediated microbubble destruction can effectively enhance the expression of bFGF gene into the infarcted myocardium of rats.It is possible to be a new methos for transfering gene and therapy of myocardial infarction.