| Objectives:1.To make theα-amanitin induced posoined mice models.2.To investigate the effects of Polysacharidum of G.Lucidum Karst on hepatic and nephritic injury induced byα-amanitin in mice.3.To investigate whether there is dose-effect relationship of the effects of the Polysacharidum of G.Lucidum Karst on hepatic and nephritic injury induced byα-amanitin in mice.Methods:1.Making the mice models:Mice models were prepared by injectingα-amanitin intraperitoneally.32 kunming male mice were randomly divided into 4 groups(8 in each):blank control group,poisoned model groups 1～3,at the beginning of the experiment,all the mice were weighed.Four groups of animals were intraperitoneally injected with 0.5ml normal saline,0.25mg/kg,0.35mg/kg,0.45mg/kgα-amanitin,respectively.The number of the deaths per day was recorded for five days.after 120 hours or after the death of the mice,the mice were dissected,a part of the liver was taken for HE staining,pathological changes were observed with light microscope.2.Experimental study:The above poisoned model group 2 was selected as the model.48 kunming male mice were randomly divided into 6 groups(8 in each):blank control group,poisoned model group,Polysacharidum of G.Lucidum Karst treatment groups 1～4,all the mice were weighed every morning.At first,the mice models treated withα-amanitin were made,4 hours later,the mice of the blank control group and poisoned model group were all intraperitoneally injected with 0.5ml normal saline,the mice of treatment groups 1～4 were respectively intraperitoneally injected with 0.5ml/kg,1.0ml/kg,2.0ml/kg,4.0ml/kg Polysacharidum of G.Lucidum Karst,once a day and for 3 days.The performance and survival status of animals were observed.The blood was collected by picking the eyeballs of the mice after 72 hours,and then the aminals were dissected,the liver and kindey were taken and weighed,the dates of the weigh were recorded for calculating organ coefficient,the cellected blood was centrifugated and the serum was collected for measuring the alanine aminotransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN),creatinine(CR).A part of the liver and kindey were taken for HE staining,pathological changes were observed with light microscope.Results:1.Making the mice models(1)Survival status:The mice of the blank control and poisoned model group 1 did not die in five days.The mice of the poisoned model group 2 began to die in the fourth day,there were six mice survival in five days.The mice of the poisoned model group 3 began to die in the second day,there were three mice survival in five days.(2)Pathological changes:The hepatic cells of the blank control group were normal.Serious liver injury happened in mice who died in model groups,the structure of hepatic lobule was unclear,cell swelling was obvious,pyknosis happened in the nucleus and macrophages increased.2.Experimental study:(1)Performance and survival status:The performance status of mice in the poisoned model group was bad,they reduced alertness and autonomic activities,also them were depressed and sleepy,compared with the blank control group,the food intake decreased.The performance status of mice in treatment groups was much better,especially in treatment group 2 and 3.The performance status of the mice in blank control group was normal.Mice of all the groups did not die in 72 hours.(2)Organ coefficients:Compared with the blank control group,the hepatic and renal organ coefficients of mice in the poisoned model groups increased(P＜0.05).Compared with these,the hepatic and renal organ coefficients decreased in the treatment groups(P＜0.05).(3)Biochemical parameters in serum:The mice of the poisoned model group manifested significant increase of ALT,AST,BUN,SCR(P＜0.01).Compared with these,ALT,AST,BUN and SCR decreased in treatment group(P＜0.05),especially in treatment group 2 and 3,the data of ALT,AST,SCR reached statistical significance between different dose treatment groups,there was dose-effect relationship of the effects of the Polysacharidum of G.Lucidum Karst on hepatic and nephritic injury induced byα-amanitin in mice.(4)Pathological changes:The hepatic cells of the blank control group were normal,clear hepatic and nephritic injury happened in mice in model group,the structure of hepatic lobule was unclear,the hepatic cells mainly manifested swelling,pyknosis happened in the nucleus and macrophages increased.Pyknosis happened in some glomerulus,swelling and necrosis happened in renal tubules,the structure of renal tubules was incomplete.Compared with the poisoned model groups,the injury was slight in the treatment group,especially in the treatment group 2 and 3.Conclusions:1.Poisoned model group 2 is suitable for this experiment(the dose of theα-amanitin is 0.35mg/kg).2.The Polysacharidum of G.Lucidum Karst have protective effects on hepatic and nephritic injury induced byα-amanitin in mice.3.There is dose-effect relationship of the effects of the Polysacharidum of G.Lucidum Karst on hepatic and nephritic injury induced byα-amanitin in mice.The efficacy of treatment group 2 and 3 is better than the other two treatment groups(the dose of the Polysacharidum of G.Lucidum Karst are 1.0ml/kg.d and 2.0ml/kg.d,respectively).
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