| Objective:To study the expression of Cyclin G1 and p53 in colorectal cancer,and its relation to the clinical pathological characteristics of colorectal cancer;to discuss the relation of p53 to the expression of Cyclin G1 in colorectal cancer;and to preliminarily evaluate the clinical significance of Cyclin G1 in the carcinogenesis and progression of colorectal cancer.Methods:We collected paraffin embedded biopsy samples of colorectal cancer tissue in 70 patients who underwent radical excision in the First Hospital of Changsha from January 2003 to June 2006(all cases have complete clinical information and no history of chemotherapy ).During the same period,we also collected paraffin biopsy samples of normal tissues from the colorectal mucosa of 31 clinically diagnosed irritable bowel syndrome(IBS) patients in the endoscopic lab.All of the cases were pathologically diagnosed.The expression of Cyclin G1 and p53 in the 70 cancer tissues and 31 normal tissues were detected using immunohistochemical SP method,such as to explore its relation to the clinical pathological characteristics of colorectal cancer,and to further discuss the correlation of Cyclin G1 and p53 in colorectal cancer.Statistical analyses were performed using SPSS 13.0 statistical software.Results:1.The positive rate of Cyclin G1 was significantly higher in the cancer tissue(62.9%,44/70 ) than that in the normal tissue(9.7%, 3/31 )(p＜0.05).The higher expression of Cyclin G1 in the cancer tissue showed significant correlation with Duke's staging(p＜0.05),but not with the factors including age,gender,histological type,and lymph metastasis(p＞0.05).2.The positive rate of p53 was significantly higher in the cancer tissue(77.1%,54/70 ) than that in the normal tissue(3.2%,1/31 ) (p＜0.05).The higher expression of p53 in the cancer tissue was not correlated with any of the following factors:age,gender,Duke's staging, histological type,and lymph metastasis(p＞0.05).3.High expression of Cyclin G1 in colorectal cancer is positively correlated with the expression of p53(rs =0.286,p＜0.05 ).Conclusion:The expressions of Cyclin G1 and p53 in colorectal cancer are significantly higher,and they are positively correlated.The over-expression of Cyclin G1 in colorectal cancer is significantly correlated with Duke's staging,suggesting Cyclin G1 may play a role in the carcinogenesis and progression of colorectal cancer. |
