Effects Of Progesterone On Expressions Of Interleukin-18 In Cerebral Cortex Of Neonatal Rats With Seizure Induced With Trifluoromethyl Ether

Object:To investigate the effects of recurrent seizures on internleukin-18 expressions in immature and mature cerebral at cortex;By Giving progesterone,investigate the change of internleukin-18 in cerebral cortex of neonatal rat which was recurrent seizure,to determine whether progesterone protects the neurons from the damage of seizures by the change of internleukin-18.Methods:96 7-day-old(PN-7d) Sprague-Daweley rats were divided randomly into three groups:The first group was control group that rats grown naturally.the second group was the seizure group,the rats were administered chemoconvulsant drug Trifluoromethyl ether(one time per day,the convulsion sustained for 30min.)in this group.The third group was the intervention group,rats of this group were administered progesterone(16mg/kg) after given Trifluoromethyl ether;ARS-13d, ARS-15days,ARS-19 days,PN-75 days later,the rats were killed and their brains processed for localization of neuron plasma antigen.The expression of interleukin-18 protein in the cortex were detected by immunohistochemistry and Western-Blot methods on ARS-1d(PN-13d), ARS-3d(PN-15d),ARS-7d(PN-19d) and PN-75d.Results:①IL-18 could be detected in the endochylema of rats cerebral cortex,The results of immunohistochemistry indicated:Compared with the control groups,the expression of IL-18 was significantly higher in the seizure groups(P＜0.05).The level of IL-18 in seizure groups rise on ARS-1d,and the level of IL-18 reached the peak on ARS-7d,then declined.But on the PN-75d the expression of IL-18 in the seizure groups also more than control groups.The intervention groups(rats were given progesterone),compared with the seizure groups,the expression of IL-18 in cortex was significantly reduce(P＜0.05).②The results of Western-Blot methods:The expression of IL-18 in cerebral cortex on the ARS-1d and ARS-3d couldn't be detected.On the ARS-7d and PN-75d.Compared the seizure groups with control group,the levels of IL-18 in the seizure groups significantly higher(P＜0.05).Compared the seizure groups with the invention groups,the levels of IL-18 in invention groups significantly reduced(P＜0.05).Conclusions:Recurrent seizure in neonatal rats rise the expression of IL-18 in cerebral cortex of rats.The progesterone could reduce the levels of IL-18 in cerebral cortex of the seizure rats;This phenomenon indicate progesterone could have anti-inflammatory effect on seizure model.It may indicate Progesterone can protect brain from injury induced by seizures in the infant rats.