Effect Of Advanced Glycation End Products On Expressions Of Hypoxia-inducible Factor-1 And Vascular Endothelial Growth Factor Protein In RF/6A Cells

ObjectiveTo investigate the effect of advanced glycation end products(AGEs) on expression of HIF-1αand VEGF protein in RF/6A cells cultured in vitro,and investigate the relationship between the expression of HIF-1αand VEGF protein.MethodsRF/6A cells were cultured in vitro and treated with AEGs and AEGs control at different concentrations(0 mg/L,50mg/L,100mg/L, 200mg/L,400mg/L,800mg/L)for 24 hours.The expression of VEGF protein was detected by ELISA,and western blot analysis was used to determine the level of HIF-1αprotein.ResultsThe expression of HIF-1αand VEGF protein was significantly higher in AEGs groups than that in AEGs control groups at different concentrations(p＜0.05).With the increasing of the concentration of AGEs,the expression of HIF-1αand VEGF protein increased and reached to the maximum at the concentration of 200mg/L,then decreased at 400mg/L and 800mg/L,but the effect wasn't shown in AEGs control groups.There was a positive correlation between the expression of HIF-1αand VEGF(p＜0.05). ConclusionsAGEs could induce the expression of HIF-1αand VEGF protein in RF/6A cells in dose-dependent manner.AGEs may raise the expression of VEGF protein by increasing the level of HIF-1αprotein,demonstrating the potential role of HIF-1α-targeted therapy in the neovascularization.