Effect Of Carbon Dioxide On The Expressions Of Survivin, PCNA, CD44v6 And The Cisplatin Sensitivity Of Human Ovarian Cancer Cell Line SKOV3

Background: Compared with the laparotomy laparoscopic operation has less trauma, less pain, rapid recovery and shorter hospital stay and becomes important way to treat benign gynecological diseases. With the development of laparoscopic operation, it's application in malignant tumor is more and more. But the argument about the safety of the operation is still very intense, especially about port-site and abdominal cavity metastasis of tumor cells. There are mangy studies about this in cellular level and animal level, and the results are very different. The mechanisms of carbon dioxide promoting the development and metastasis of malignant tumor cells are also still uncertain. There are various theories in nation and abroad, such as acidosis, immunologic injury , physical damage and transfer agent and adhesion molecule. But reports in cellular level about sensitivity to chemotherapeutic drugs of malignant tumor cells after treated with carbon dioxide are rare.Objective: To explore the effect of carbon dioxide on the expressions of Survivin, PCNA, CD44v6 and the cisplatin sensitivity of human ovarian cancer cell line SKOV3. Methods: Human ovarian cancer cell line SKOV3 was used. Cells at logarithmic growth phase were incubated in culture flask. After 24 hours, the cells were divived into four groups: control, DDP, CO₂, CO₂+DDP. The cells in control group were cultured in incubator continually for 72 hours. DDP group cells were cultured in culture medium containing DDP (10μg/ml) after another 24 hours. CO₂ group cells were put in analogous CO₂ pneumoperitoneum environment for 3 hours and then were cultured for 72 hours. CO₂+DDP group cells were cultured in culture medium containing DDP (10μg/ml) 24 hours later after treated with CO₂ and then were cultured for 48 hours. RT-PCR and immunocytochemical staining were used to examine the expressions of Survivin, PCNA, CD44v6 of these cells at mRNA and protein levels. Data were analyzed with SPSS for Windows 13.0 statistical software. Statistical method was analysis of variance of factorial design and t-test. P<0. 05 was considered to be significant.Results: There was no interaction between CO₂ and DDP (P>0. 05) . Compared with control group, the expressions of Survivin, PCNA, CD44v6 of CO₂ group cells increased (P<0. 01) . The expressions between all DDP groups and no DDP groups are significantly different with lower Survivin, PCNA, CD44v6(P<0. 01). The expressions of CO₂+DDP group cells had statistical significance group but had no statistical significance compared with control group (P>0. 05) .Conclusion : CO₂ pneumoperitoneum can promote the development and metastasis of malignant ovarian tumor cells by significantly increasing the expressions of Survivin, PCNA, CD44v6 at mRNA and protein levels, and cisplatin can cansel out the effects of CO₂ pneumoperitoneum.