| Objective:1.To establish the Bleomycin-induced pulmonary fibrosis model in rats; 2.To observe the effects of Huayulifei on the pulmonary fibrosis in rats; 3.To study the effects of Huayulifei on expressions of MMP-2,TIMP-1 in lung tissue of rats; 4.To study the differences of Huayulifei and Hydrocortisone sodium succinate on pulmonary pathology, expressions of MMP-2,TIMP-1 lung tissue of rats.Methods:84 Male Wistar rats were randomly divided into control group (21, saline+saline), model group (21, Bleomycin+saline), Huayulifei group(21, Bleomycin+Huayulifei) and hydrocortisone group (21, Bleomycin+ Hydrocortisone sodium succinate).The rats were anesthetized with abdominal injection of pentobarbital sodium in a dose of 35mg/kg body weight and then were intratracheally dripped with Bleomycin hydrochloride (5mg/kg in 0.25ml saline) in experimental groups, while 0.9% saline in control group. Treatments were started from the next day with a daily oral Huayulifei (17190mg/kg) in Huayulifei group, intraperitoneal Hydrocortisone sodium succinate (25mg/kg) in Hydrocortisone group, and oral saline in control group and model group, respectively.Rats were killed at7, 14, and 28 days after Bleomycin, lungs isolated for Hematoxylin and eosin-staining, and the expression of MMP-2 and TIMP-1 protein was measured by immunohistochemical technique.Results:1.General observation of rats: Eighty-four rats were involved in the analysis. The action, body figure, inspiration and bowels of rats were well in control group. The inaction, dyspnea, loss of weight and out of spirit of rats were found in model group. The rats'manifestations were similar to those in model group at early stage, but the situation turn better after being treated with Huayulifei 7 days. There were no significant difference between Hydrocortisone group and Huayulifei group.2.General observation of lung:There were no abnormal findings of the majority ratsin control group, whose lung profile was clear, smooth, pink and elastic. The dense bit haemorrhage, focal bleeding, nodule change and lobe volume shrink were found in model group.The findings of lung morphology in Huayulifei group were similar to those in control group.The findings of lung morphology in hydrocortisone group were similar with those in Huayulifei group.3. Alveolitis and fibrosis:There were completely normal parenchymal structures in control group.The alveolar septums and pleura were thin, and the alveolar walls were formed in a fine pattern.There were moderate alveolitis accumulated in lung tissue at day 7. There were severe alveolitis manifested by a thickening of alveolar walls accompanied by an accumulation of inflammatory cells within the parenchymal structures in model group at dayl4.There were moderate or severe fibrosis manifested by a thickening of alveolar walls accompanied by an accumulation of fibroblast, collagen within the parenchymal structures in model group at day28. The alveolitis and pulmonary fibrosis in Huayulifei group was alleviated significantly compared with those in model group.The effect was not significant when hydrocortisone group compared with Huayulifei group.4.Expressions of MMP-2 and TIMP-1 : MMP-2 were weakly expressed in cytoplasm of vascular endothelial cell,pulmonary alveolar macrophage and a few bronchiole mucosa epithelial cell.in control group;MMP-2 were remarkably expressed in cytoplasm of alveolar macrophages, epithelial cells and endothelial cells fibroblasts in model group;The expression of MMP-2 were significantly decreased in huayulifei group at day7,14 and 28, the effect was not significant when hydrocortisone group compared with Huayulifei group. TIMP-1 were weakly expressed in control group, they were expressed in cytoplasm of epithelial cells, alveolar macrophages, fibroblasts and collagen in model group. The expression of TIMP-1 were significantly decreased in huayulifei group at day7, 14 and 28.Conclusions:1. Huayulifei can ameliorate bleomycin-induced pulmonary fibrosis and alveolitis inrats;2. Huayulifei may partly work by modulating the expression of MMP-2 and TIMP-1 in lung tissue;...
|
PDF Full Text Request