Comparative Study On The Effect Of Hydrogen Peroxide And Glutathione On Proliferation Of Type Ⅰ And Type Ⅱ Endometrial Cancer Cells

Objective To compare the ability of typeâ… and typeâ…¡endometrial cancer cells to resist oxidative stress and the effect of glutathione on proliferation of typeâ… and typeâ…¡endometrial cancer cells and investigate the related mechanisms.Methodâ‘ The typeâ… and typeâ…¡endometrial cancer cells were challenged by H₂O₂ or GSH, and the proliferation of the cells was observed with the MTT assay.â‘¡The typeâ… and typeâ…¡endometrial cancer cell were treated with H₂O₂ or GSH, respectively,and the cellular ROS,GSH and GSSG levels and SOD,CAT and GPx activities were determined.Resultsâ‘ Without the treatment with H₂O₂ or GSH,the typeâ… endometrial cancer cell, compared to typeâ…¡endometrial cancer cell,had the lower ROS level and CAT activitiy and the higher GSH level,GSH/GSSG ratio and SOD and GPx activities.â‘¡In the condition of oxidative stress,the proliferation was inhibited in both types of endometrial cancer cells,especially in typeâ… endometrial cancer cells.In the condition of treating the cells with reductive GSH,the proliferation was promoted in typeâ… endometrial cancer cells while inhibited in typeâ…¡endometrial cancer cells. â‘¢In the condition of oxidative stress,the ROS level increased in both typeâ… and typeâ…¡endometrial cancer cells.The GSH level,GSH/GSSG ratio and SOD and CAT activities reduced in the typeâ… endometrial cancer cell while the GSH and GSSG levels,GSH/GSSG ratio and SOD,CAT and GPx activities increased in the typeâ…¡endometrial cancer cell.â‘£In the condition of treating the cells with reductive GSH,the ROS level decreased in both typeâ… and typeâ…¡endometrial cancer cells.The GSH level,GSH/GSSG ratio and SOD,CAT and GPx activities reduced in the typeâ… endometrial cancer cell while the GSH and GSSG levels and CAT and GPx activities decreased and GSH/GSSG ratio increased markably in the typeâ…¡endometrial cancer cell.Conclusionâ‘ The typeâ… endometfium cancer cell,compared to typeâ…¡endometrium cancer cell,had the lower ROS level and CAT activitiy and the higher GSH level, GSH/GSSG ratio,and SOD and GPx activities.â‘¡The typeâ… and typeâ…¡endometrial cancer cells showed different properties in the proliferation after they were challenged by H₂O₂ and GSH.The proliferation of both types endometrial cancer cells was inhibited by H₂O₂.The proliferation of typeâ…¡endometrial cancer cells was inhibited by reductive GSH while that of typeâ… endometrial cancer cells was promoted in the same condition.â‘¢The GSH oxidation-reduction system and antioxidase system in the typeâ… endometrial cancer cell cannot effectively response to oxidative stress,and the cell is sensitive to oxidation and damaged easily.The exogenetic reductive GSH can reduce the level of intracellular oxidative stress and promote the proliferation of typeâ… endometrial cancer cell.â‘£The GSH oxidation-reduction system and antioxidase system in the typeâ…¡endometrial cancer cell can effectively response to the exogenetic oxido-reduction stress,and the cell is not damaged easily by the oxidative stress.