The Effect Of Atorvastatin On The Change Of Mesenteric Artery Smooth Muscle Cellular NADPH Oxidase-active Oxygen Species Signal Pathway And Proliferation Induced By Angiotensin Ⅱ In Hypertensive Patients

objective: To observe change of angiotensinâ…¡- NADPH oxidase - active oxygen signal pathway and proliferation in non-hypertensive and hypertensive patients'mesenteric artery smooth muscle cells . At the same time, we also observe effect of atorvastatin on this signal pathway and smooth muscle cellular proliferation. Methods: 1. Cell culture and identification: human mesenteric artery smooth muscle cells were isolated and cultured by organization paste blocks in vitro, and identified by inverted optical microscope and immunocytochemical method. 2. Testing methods: smooth muscle cellular proliferatial rate was determined by MTT assay; reactive oxygen species was detected with flow cytometry by dihydrochloride acetyl acid dichloride fluorescent yellow (DCFH-DA) as a fluorescent probe of the active oxygen; cells p22phox mRNA expression changes was tested by reverse transcription-polymerase chain reaction (RT-PCR). 3. Experimental groups: non-hypertension and hypertension was divided into two major groups, then each major group was divided into the control group, adding the concentration of 10-6 mol/L angiotensinâ…¡incubation group, joined concentration of 10-6mol/L angiotensinâ…¡and atorvastatin calcium incubation group. Results: 1. The concentration of angiotensinâ…¡10-6 mol/L angiotensinâ…¡promote the mesenteric artery smooth muscle cellular proliferation. Compared with the control group, OD value of only adding angiotensinâ…¡drug sub-group were significantly increased (P <0.05).2. Angiotensinâ…¡in the concentration of 10-6 mol/L can promote formation of reactive oxygen species the mesenteric artery smooth muscle cells. Flow cytometry show that fluorescence intensity ratio of only adding angiotensinâ…¡drug subgroup was significantly higher than the control group (P <0.05 ).3. Angiotensinâ…¡in the concentration of 10-6mol/L promote the mesenteric artery smooth muscle cellular p22phox mRNA expression. 4. Compared with non-hypertensive patients, angiotensinâ…¡- NADPH oxidase - active oxygen pathway of hypertensive patients mesenteric artery smooth muscle cells greatly promote proliferation. The result show that proliferation rate of cells and the cellular p22phox mRNA expression were significantly increased (P <0.05). 5. Atorvastatin can inhibit angiotensinâ…¡- NADPH oxidase - the active oxygen signal conduction pathway and decrease proliferation. In the atorvastatin incubation group , the rate of cellular proliferation, cellular reactive oxygen species and the cellular p22phox mRNA expression are lower than the concentration of 10-6mol/L angiotensinâ…¡incubation group (P <0.05).Conclusions: 1. Human mesenteric artery smooth muscle proliferation induced by angiotensinâ…¡may party get access to the angiotensinâ…¡- NADPH oxidase - active oxygen species pathway. 2. Angiotensinâ…¡- NADPH oxidase - active oxygen species pathway, by comparison, plays a more significant role in the hypertensive patients mesenteric artery smooth muscle cellar proliferation. 3. Atorvastatin can inhibit angiotensinâ…¡- NADPH oxidase - the active oxygen signal conduction pathway. This may be one of ways of atorvastatin inhibiting artery smooth muscle proliferation.