Synthesis And Properties Of Hydrogels Based On Water Soluble Chitosan Derivative

Bond tissue engineering has developed quickly since the middle of 20 century, especially in recent few years. Chitosan is currently receiving a great deal of interest for biomedical and tissue engineering due to its biocompatibility, biodegradability, antibacterial and wound-healing activity. However, the application of chitosan is usually limited for low soluble property which was caused by intra- and/or intermolecular hydrogen bonding.In this paper, a water soluble chitosan derivative which is (methacryloyloxy) ethyl carboxyethyl chitosan (CS-EGAMA) was synthesized by Michael addition and two kinds of gels for tissue engineering were prepared by photopolymerization technique. The main contents and conclusions are described as follows.1. Photocrosslinkable chitosan hydrogels based on water-solubleCS-EGAMA and Polyethylene glycol diamethacrylate (PEGDMA).The characterization was studied by FTIR, TG, DSC SEM, swell test, XRD, cytotoxicity and cellular compatibility. The results indicated that the hydrogels were sensitive to pH of the medium, no cytotoxicity for L929 and SW1353, satisfactory for the composite to be used in bioapplications.2. Photocrosslinkable chitosan hydrogels based on CS-EGAMA, Polyethylene glycol diacrylate (PEGDA) and N,N-Dimethylacrylamide (DAA). The characterization was studied by FTIR, TG, DSC SEM, swell test, DMA, cytotoxicity and cellular compatibility. The results indicated that the hydrogels were crosslink in 8min with 30mW/cm~2 condition and 90% double bond conversion, good heat stability and strength. The hydrogel could be used as a tissue engineered scaffolds for cell transplant.