Influence Of YiShenHuaYu On The Expression Of Platelet Derived Growth Factor BB In Rat With Renal Interstitial Fibrosis

Objectives: To set up rat models of Renal Interstitial Fibrosis. To investigate the protective influence of YiShenHuaYu(YSHY) on Serum Creatinine and Blood Uria Nitrogen and 24-hour Urine Protein, and the expression of Platelet Derived Growth Factor BB(PDGF-BB) in the Rat of Renal Interstitial Fibrosis. To disscuss some mechanisms of treating the Rat with Renal Interstitial Fibrosis.Methods: The rats were randomly divided into sham-opration group, model group, Benazepril treatment group, YSHY high-dose and low-dose treatment group. Except sham-opration group, the other four groups were induced by unilateral ureteral obstruction (UUO) to set up RIF models. The rats were sacrificed at 3, 7, 14, 21 and 28 day. The change of Blood Uria Nitrogen was observed by using Two acetyl-oxime method, The change of Serum Creatinine was observed by using Picric acid method, The change of 24-hour Urine Protein was observed by using Colorimetry method, The alterations of renal constitution were detected by using the technique of Histopatholo- gy(the Haematoxylin and Eosin Staining,Masson Staining). The expressions of PDGF-BB in the kidneys were observed with theβ-actin as intrinsic control by using Western Blot. Meanwhile, the influences of YSHY on the above changes were studied.Results: Compared to Sham-operation group, the level of serum creatinine and blood uria introgen was elevated gradually in rat models with P<0.01. Nonvisible changes can be seen on 24-hour urine protein. There were gradually serious ectatic and atrophy tubules, inflammatory cell infiltration and interstitial fibrosis. The expression of PDGF-BB was increased with the development of renal interstitial fibrosis from day 3 after UUO, and then gradually increased. Compared to models, all the changes mentioned above such as renal function, renal histopathologity were improved in the groups after given drugs. Mealwhile, Compared to models,all the indicators mentioned above were improved after given drugs with P<0.05 or P<0.01.Conclusion: The rat model of RIF was established successfully which was confirmed through biochemistry and renal histopathological alteration. YSHY can improve the renal functions and renal constitution. RIF may be alleviated by decreasing expression of PDGF-BB. Although there is still much work to be done, we can draw a conclusion that YSHY can be used to treat Chronic Renal Failure.