The Proliferation Suppression Effect Of Combination Of Magnesium Sulfate Infusion And Oxaliplatin On Human Gastric Cancer Cell Line SGC-7901 In Vitro

BACKGROUND: Chemotherapy is important in treating gastric cancer. Past studies suggested that Progression-Free Survival (PFS) and Overall Survival (OS) can be prolonged by chemotherapeutic treatment of advanced gastric cancer. Oxaliplatin is the third-generation platinum. Oxaliplatin is active in colorectal cancer,gastricc ancer,lung cancer et al. Neuro-toxicity is its dose-limiting toxicity, Disabling peripheral neuropathy has a significant negative impact on quality of life and interrupt the chemotherapy.Accordingly, potential neuroprotective agents are to be investigated. Sevevl studies suggested that Magnesium sulfate infusion is a hopeful chemoprotectant to ameliorate the toxicity associated with platinum-based chemotherapeutic agent. There have been few studies that neuroprotective effect of Magnesium sulfate infusion in experiment of neurotoxicity of Oxalplatin in vitro.The aim of this studies was to investigate whether the toxicity of Oxaliptin reduced by Magnesium sulfate infusion.METHODS: 1. SGC-7901 cells were cultured with Oxaliplatin and Magnesium sulfate infusion at different concentrations after 24 hours, methyl thiazolyl tetrazolium (MTT) was used to measure the anticancer activity. 2. The ability of Oxaliplatin alone, Magnesium sulfate infusion alone and Oxaliplatin in combination with Magnesium sulfate infusion to induce apoptosis was also measured by a flow cytometer.RESULTS: 1. Magnesium sulfate infusion intensified the sensitivity of Oxaliplatin induced apoptosis, but this effect was not significant at 0.40mg/ml of magnesium sulfate infusion. 2. Magnesium sulfate infusion at 10.00mg/ml level clould increace the sensitivity of Oxaliplatin induced apoptosis ,which was measured by a flow cytometer.CONCLUSIONS: In this study, Magnesium sulfate infusion (50.00mg/ml,10.00mg/ml,2.00mg/ml) intensified the sensitivity of Oxaliplatin induced apoptosis,but this effect was not significant at 0.40mg/ml of Magnesium sulfate infusion. And they can be applied to animal experiment to further assess the effect.Magnesium sulfate infusion was not reduce the anti-tumor effect of Oxaliplatin,which provide a theoretical basis for the clinical application of magnesium sulfate infusion..