Effect Of Intermittent Administration Of Nitroglycerin On BMD And Serum NOS Levels In Glucocorticoid-Induced Osteoporosis

Objective To observe the effect of NG on serum NOS levels in rat model with glucocorticoid-induced osteoporosis and its effectiveness on preventing glucocorticoid-induced osteoporosis.Methods Thirty male SD rats, weighted (251±12.5g) each, were randomly divided into 3 groups (10 rats for each group). In the experiment group A rats were given natrium chloride and dexamcthasone .In the group B were given dexamcthasone and NG, .In the control group C rats were given only natrium chloride. Eight weeks later, the bone mineral density of the third lumbar vertebral bodies and the femur of all the rats were measured, and the structure of bone trabeculae, the body weight, NOS, ALP in serums were measured too.Results The values of bone mineral density in Group C were significantly higher than that of Group B,(P<0.01).No significant difference was observed in bone mineral density between Group A and Group C (P>0.05). The bone mineral density of femur between the there groups were no significant difference .The structure of bone trabeculae of Group C was similar to that of Group B, and Group A presented the feature of osteoporosis. ALP levels of Group A were significantly increased compared to the corresponding value of Group B and Group C(P<0.01) The serum NOS between the three groups was no significant difference (P>0.05)Conclusion The change of serum NOS is not suitable for evaluating the bone loss in rat model with glucocorticoid-induced osteoporosis. Intermittent administration of NG can prevent the osteoporosis induced by gulcocorticoid effectively.