The Study Of Radiosensitizing Effect On Gastric Carcinoma Cells By Arsenic Trioxide

ObjectiveTo study the radio-sensitizing effect of arsenic trioxide (As₂O₃) in human gastric carcinoma cells.MethodsAs human gastric carcinoma cells SGC-7901 selected for study.All experiments were done three times,and we took the mean as the final result.â‘ The toxic effect of As₂O₃ was examined to define the IC50 according to MTT assay in SGC-7901 cells,and the q value was calculated through the three group.â‘¡They were divided into four groups,such as control group,medication group,irradiation group and As₂O₃+ irradiation group.Cell survival rate was calculated by cloning assay,and single-hit multitarget model was used to plot the survival curve which could evaluate the effect of cell killing.Also we got Do,Dq,SER valu from it.ResultsThe IC50 in SGC-7901 cells was 12.65 umol/L. Inhibition of cell proliferation seemed more dependent on the increase of As₂O₃ concentration.Compared to medication group and irradiation group, As₂O₃+ irradiation group could significantly inhibit the cell proliferation of SGC-7901 cells.And the result demonstrated the synergism effect between As₂O₃ and irradiation when they were both used to kill the cells.The Do and Dq value were 2.75Gy and 2.52Gy in irradiation group.When As₂O₃ was administrated at 2 umo/L and 5 umol/L with irradiation,their Do,Dq,SER(Do) and SER(Dq) were 2.18Gy,1.89Gy,1.26,1.33 and 1.78Gy,1.72Gy,1.54,1.51.ConclusionsAs₂O₃ was shown as possessing a radiosensitizing effect in vitro in SGC-7901 gastric carcinoma cells according MTT assay and colony-forming assay. So the study was supposed to provide experimental foundation that arsenic trioxide as a radiosensitizer could be used for the therapeutic alliance of gastric cancer.