Biocompatibility Of PBTEOLA Copolymers With Skin-derived Precursor Cells

Objective: to explore the biocompatibility of poly(1,4-butylene terephthalate-co- ethylene oxide-co-DL-lactide) (PBTEOLA) copolymers with skin-derived precursor cells and investigate the possibility of PBTEOLA copolymers being used as scaffold in tissue engineering.Methods: Skin-derived precursor cells were separated and cultured with PBTEOLA copolymers in vitro. Cells were examined by inverted microscope, scanning electron microscope (SEM), HE staining and staining of DAPI to assess cell growth and morphology. Cell adhesion and proliferation were detected with MTT assay.Results: The SKPs grew adherently 24 hours after plantation on the surface of PBTEOLA copolymer, and reached a growth peak after 5 to 7 days. HE stain and SEM scanning revealed that SKPs adhered on the surface and pore of copolymer, and linked together with pseudopods and excreted cellular matrix. MTT method showed that copolymer had no significantly negative effect on the growth of SKPs (p>0.05). Growth curves of the cells in the experimental and control groups were alike and there was no difference statistically (p>0.05).Conclusion: PBTEOLA copolymer has a good biocompatibility with skin-derived precursor cells and has a potential to be used as scaffold in tissue engineering.